High prevalence of vanB2 vancomycin-resistant Enterococcus faecium in Taiwan

J. J. Lu, C. L. Perng, M. F. Ho, T. S. Chiueh, W. H. Lee

研究成果: 雜誌貢獻文章同行評審

21 引文 斯高帕斯(Scopus)


Thirty-six VanB glycopeptide-resistant Enterococcus faecium isolates were collected from patients in five different hospitals in Taiwan. The vancomycin resistance genes were amplified by the long vanB PCR, which amplifies the 6,373-bp vanB gene cluster including the vanRB2, vanSB2, vanYB2, vanWB2, vanHB2, vanB2, and vanXB2 genes. The deduced amino acid sequences were found to be 95 to 98% homologous to those of the vanB1 gene cluster: VanRB1, 97%; VanSB1, 97%; VanYB1, 96%; VanHB1, 95%; VanB1, 96%; and VanXB1, 98%. Restriction enzyme analysis of the long vanB PCR products revealed that all 36 isolates had the same vanB2-specific pattern. DNA sequence analysis of the vanB2 gene, which is a D-Ala-D-Lac ligase gene, revealed that none of the 36 sequences were identical to the previously published vanB2 sequence. Thirty-one isolates had 1 nucleotide different from the published vanB2 sequence. The sequences of the other five isolates differed from the published vanB2 sequence by 2 or 3 nucleotides. Four isolates with a low or moderate resistance to vancomycin (MIC = 4 to 32 μg/ml) were found to have the same leucine-to-methionine change at amino acid position 308 of the vanB2 gene. The genomic DNAs of all 36 isolates were digested with SmaI and then typed by pulsed-field gel electrophoresis (PFGE). Eight different PFGE types (I to VIII) were observed, and type I was found to be prevalent in all hospitals examined in this study. This result suggests that intra- and interhospital dissemination of this E. faecium strain has occurred in Taiwan.

頁(從 - 到)2140-2145
期刊Journal of Clinical Microbiology
出版狀態已發佈 - 2001

ASJC Scopus subject areas

  • 微生物學(醫學)


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