Glutamine modulates sepsis-induced changes to intestinal intraepithelial γδT lymphocyte expression in mice

Wan Yun Lee, Ya Mei Hu, Tsui Ling Ko, Sung Ling Yeh, Chiu Li Yeh

研究成果: 雜誌貢獻文章同行評審

25 引文 斯高帕斯(Scopus)


This study investigated the effect of glutamine (GLN) on intestinal intraepithelial lymphocyte (IEL) γδT-cell cytokines and immune regulatory factor gene expressions in a mouse model of polymicrobial sepsis. Mice were randomly assigned to a normal group, a sepsis with saline (SS) group, or a sepsis with GLN (SG) group. All mice were fed a chow diet. Sepsis was induced by cecal ligation and puncture (CLP). The SS group was injected with saline, and the SG group was given 0.75 g GLN/kg body weight once via a tail vein 1 h after CLP. Septic mice were killed 12 h after CLP, and IEL γδT cells of the animals were isolated for further analysis. Results showed that compared with normal mice, sepsis resulted in lower IEL γδT-cell percentage and higher messenger RNA expressions of interferon γ, tumor necrosis factor α, interleukin 4 (IL-4), IL-13, IL-17, retinoid acid receptor-related orphan receptor γt, and complement 5a receptor by IEL γδT cells. These immunomodulatory mediator genes exhibited decreases, whereas IL-7 receptor expression increased in IEL γδT cells in septic mice with GLN administration. Annexin V/7-amino-actinomycin D stain revealed significantly lower rates of apoptosis, and IEL γδT-cell percentage was higher in the SG group. The histological findings also showed that damage to intestinal epithelial cells was less severe in the SG group. These results indicated that a single dose of GLN administered as treatment after the initiation of sepsis prevented apoptosis of IEL γδT cells and downregulated γδT cell-expressed inflammatory mediators that may consequently ameliorate the severity of sepsis-induced intestinal epithelial injury.
頁(從 - 到)288-293
出版狀態已發佈 - 8月 2012

ASJC Scopus subject areas

  • 重症監護和重症監護醫學
  • 急診醫學


深入研究「Glutamine modulates sepsis-induced changes to intestinal intraepithelial γδT lymphocyte expression in mice」主題。共同形成了獨特的指紋。