Glutamine Administration Modulates Endothelial Progenitor Cell and Lung Injury in Septic Mice

Man Hui Pai, Yao Ming Shih, Juey Ming Shih, Chiu Li Yeh

研究成果: 雜誌貢獻文章同行評審

19 引文 斯高帕斯(Scopus)


ABSTRACT: This study investigated the effects of glutamine (GLN) administration on circulating endothelial progenitor cells (EPCs) and lung angiopoietin (Ang) gene expressions in polymicrobial sepsis. Mice were randomly assigned to a normal control group (NC), septic saline group (SS), and septic GLN group (SG). All mice were fed with a chow diet. Sepsis was induced by cecal ligation and puncture (CLP). The mice in SS group were injected with saline whereas SG group administered 0.75?g GLN/kg body weight once via tail vein 1?h after CLP. Mice were sacrificed 24, and 48?h post-CLP. Their blood and lungs were collected for further analysis. The results showed that, compared to normal mice, sepsis resulted in higher C-X-C motif chemokine-12, vascular endothelial growth factor, nitric oxide levels and a higher circulating EPC percentage. Also, inflammatory cytokine concentrations and Ang-2 gene expression were upregulated in lung tissues. GLN administration enhanced the mobilization of EPC, downregulated inflammatory cytokine production and the Ang-2 gene expressions in lungs. Histopathological findings showed that, the extent of inflammatory lesions of the lung alveolar was less severe in the SG group than the SS group after CLP. Our results suggest that a single dose of intravenous GLN administration after initiation of sepsis promote the mobilization of circulating EPC and modulates the balance of Ang-Tie2 axis that may improve the vascular function, ameliorate inflammation and protect lung injury against polymicrobial sepsis.
頁(從 - 到)587-592
出版狀態已發佈 - 10月 1 2016

ASJC Scopus subject areas

  • 重症監護和重症監護醫學
  • 急診醫學


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