TY - BOOK
T1 - Global Transcriptomics and Proteomics Analyses for Biomarker Identification and Validation in Head and Neck Squamous Cell Carcinoma
AU - Chi, Li-Hsing
N1 - null
VL - 博士
PY - 2022
Y1 - 2022
N2 - Head and neck squamous cell carcinoma (HNSCC) represents a significant health concern worldwide. Surgery and chemo-radiotherapy are still the standard of care for HNSCC patients. The breakthrough improvement of those interventions should depend on 1) adequate surgical margin and radiotherapy guided by pathology, 2) predictive biomarkers, which are guiding a systemic therapy, and 3) holistic cancer care. The survival analysis could conclude the manifest of clinical and multiple omics data to find high-impact biomarkers. To discover cancer biomarkers, we use mass spectrometry method to analyze HNSCC. Traditionally, differential gene expression in HNSCC has been exploited to detect biomarkers.Global gene expression incorporated with survival analysis of HNSCC are employed for the first time in 2021, to the best of our knowledge.Data retrieval and pre-processing, feature selection, sliding-window cutoff selection, Kaplan—Meier estimate, and Cox proportional hazard regression model are all part of the "pvalueTex" in-house workflow. It could be utilized to find predictive biomarkers of surgical margins and to provide holistic cancer therapy. Four validated biomarkers (TMSB4X, CAMK2N1, CALML5, and FCGBP), clinical tumor size, and surgical margin status all are independent prognosis factors in HNSCC. Overexpression of TMSB4X is associated with a poor prognosis of overall survival (OS) and recurrence-free survival (RFS) of those patients. TMSB4X knockdown in the HNSCC cell line reduced proliferation, invasion, and cervical lymph node metastasis in vitro and in vivo. More- over, CAMK2N1, CALML5, and FCGBP, are also substantially linked with patients’ OS. Holistic cancer care considers the importance of the spirit, emotion, body, and social relationships. Ultimately, holistic cancer therapy will be a comprehensive tailored personalized medication.
AB - Head and neck squamous cell carcinoma (HNSCC) represents a significant health concern worldwide. Surgery and chemo-radiotherapy are still the standard of care for HNSCC patients. The breakthrough improvement of those interventions should depend on 1) adequate surgical margin and radiotherapy guided by pathology, 2) predictive biomarkers, which are guiding a systemic therapy, and 3) holistic cancer care. The survival analysis could conclude the manifest of clinical and multiple omics data to find high-impact biomarkers. To discover cancer biomarkers, we use mass spectrometry method to analyze HNSCC. Traditionally, differential gene expression in HNSCC has been exploited to detect biomarkers.Global gene expression incorporated with survival analysis of HNSCC are employed for the first time in 2021, to the best of our knowledge.Data retrieval and pre-processing, feature selection, sliding-window cutoff selection, Kaplan—Meier estimate, and Cox proportional hazard regression model are all part of the "pvalueTex" in-house workflow. It could be utilized to find predictive biomarkers of surgical margins and to provide holistic cancer therapy. Four validated biomarkers (TMSB4X, CAMK2N1, CALML5, and FCGBP), clinical tumor size, and surgical margin status all are independent prognosis factors in HNSCC. Overexpression of TMSB4X is associated with a poor prognosis of overall survival (OS) and recurrence-free survival (RFS) of those patients. TMSB4X knockdown in the HNSCC cell line reduced proliferation, invasion, and cervical lymph node metastasis in vitro and in vivo. More- over, CAMK2N1, CALML5, and FCGBP, are also substantially linked with patients’ OS. Holistic cancer care considers the importance of the spirit, emotion, body, and social relationships. Ultimately, holistic cancer therapy will be a comprehensive tailored personalized medication.
M3 - Doctoral Thesis
PB - Taipei Medical University
CY - Taipei
ER -
