Formalin fixation alters water diffusion coefficient magnitude but not anisotropy in infarcted brain

Shu Wei Sun, Jeffrey J. Neil, Hsiao Fang Liang, Yong Y. He, Robert E. Schmidt, Chung Y. Hsu, Sheng Kwei Song

研究成果: 雜誌貢獻文章同行評審

176 引文 斯高帕斯(Scopus)

摘要

This study was designed to determine whether formalin fixation alters diffusion parameters in the infarcted brain. Diffusion tensor images were obtained from anesthetized mice 1 hr after middle cerebral artery occlusion and repeated after formalin fixation of brains. In live animals, there was a significant decrease in the trace of the diffusion tensor (Tr(D)) in infarcted cortex and external capsule compared with contralateral brain areas, with no change in relative anisotropy (RA). After formalin fixation, Tr(D) was reduced 30-80%. However, the Tr(D) differential present in vivo between injured and healthy tissues was lost, with Tr(D) reduced to similar values in all tissues except for the edge of the cortical infarction, where it was lower than in surrounding tissues. RA values were unchanged after fixation. This study supports the preservation of diffusion anisotropy for both healthy and injured white matter in fixed mouse brain. However, the sensitivity of water diffusion in detecting tissue injury in vivo is not preserved in fixed tissues.
原文英語
頁(從 - 到)1447-1451
頁數5
期刊Magnetic Resonance in Medicine
53
發行號6
DOIs
出版狀態已發佈 - 6月 2005

ASJC Scopus subject areas

  • 放射學、核子醫學和影像學
  • 放射與超音波技術

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