TY - JOUR
T1 - Extraction method development for nanoplastics from oyster and fish tissues
AU - Chang, Yu Shan
AU - Chou, Shih Hsuan
AU - Jhang, Ya Jhu
AU - Wu, Tai Sing
AU - Lin, Li Xin
AU - Soo, Yun Liang
AU - Hsiao, I. Lun
N1 - Funding Information:
This work was supported by the Ministry of Science and Technology (MOST), Taiwan (107-2218-E-038-006-MY3) and Taipei Medical University (TMU), Taiwan (TMU106-AE1-B27). The authors acknowledge the academic and science graphic illustration service provided by TMU Office of Research and Development.
Funding Information:
This work was supported by the Ministry of Science and Technology (MOST), Taiwan ( 107-2218-E-038-006-MY3 ) and Taipei Medical University (TMU), Taiwan ( TMU106-AE1-B27 ). The authors acknowledge the academic and science graphic illustration service provided by TMU Office of Research and Development.
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2022/3/25
Y1 - 2022/3/25
N2 - Nanoplastics are now found in some environmental media and consumer products. However, very limited data on nanoplastics are available for one of the main human consumption sources of microplastics: seafood. Unlike microplastics, a method for extracting nanoplastics from seafood is still lacking. Herein, a combination of common extraction techniques including enzymatic digestion, sequential membrane filtration, centrifugal concentration, and purification (dialysis and sodium dodecylsulfate (SDS) incubation), was developed to extract nanoplastics from oyster and fish tissues. Corolase with subsequent lipase treatment achieved the highest digestion efficiencies (88– 89%) for non-homogenized tissues compared to other proteases and additional cellulase or H2O2 treatment. With the exception of polyethylene terephthalate (PET), enzymatic digestion did not change the morphology or structure of polyvinyl chloride (PVC), polyethylene (PE), or polystyrene (PS) nanoplastic particles, and the subsequent extraction procedures had good recoveries of 71– 110% for fluorescence-labeled 76-nm PVC and 100- and 750-nm PS, as validated by a Nanoparticle Tracking Analysis (NTA). Few of the 1011 digested residual particles of 150– 300 nm in diameter per oyster or per serving of fish tissue were left in the method blank. Consequently, this efficient approach could be used as a pretreatment protocol for current potential nanoplastic detection methods.
AB - Nanoplastics are now found in some environmental media and consumer products. However, very limited data on nanoplastics are available for one of the main human consumption sources of microplastics: seafood. Unlike microplastics, a method for extracting nanoplastics from seafood is still lacking. Herein, a combination of common extraction techniques including enzymatic digestion, sequential membrane filtration, centrifugal concentration, and purification (dialysis and sodium dodecylsulfate (SDS) incubation), was developed to extract nanoplastics from oyster and fish tissues. Corolase with subsequent lipase treatment achieved the highest digestion efficiencies (88– 89%) for non-homogenized tissues compared to other proteases and additional cellulase or H2O2 treatment. With the exception of polyethylene terephthalate (PET), enzymatic digestion did not change the morphology or structure of polyvinyl chloride (PVC), polyethylene (PE), or polystyrene (PS) nanoplastic particles, and the subsequent extraction procedures had good recoveries of 71– 110% for fluorescence-labeled 76-nm PVC and 100- and 750-nm PS, as validated by a Nanoparticle Tracking Analysis (NTA). Few of the 1011 digested residual particles of 150– 300 nm in diameter per oyster or per serving of fish tissue were left in the method blank. Consequently, this efficient approach could be used as a pretreatment protocol for current potential nanoplastic detection methods.
KW - Enzymatic digestion
KW - Nanoparticle tracking analysis
KW - Nanoplastics
KW - PVC
KW - Recovery
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U2 - 10.1016/j.scitotenv.2021.152675
DO - 10.1016/j.scitotenv.2021.152675
M3 - Article
C2 - 34968609
AN - SCOPUS:85122304109
SN - 0048-9697
VL - 814
JO - Science of the Total Environment
JF - Science of the Total Environment
M1 - 152675
ER -