Expression, purification, and characterization of anti-Zika virus envelope protein: Polyclonal and chicken-derived single chain variable fragment antibodies

Pharaoh Fellow Mwale, Chi Hsin Lee, Liang Tzung Lin, Sy Jye Leu, Yun Ju Huang, Liao Chun Chiang, Yan Chiao Mao, Yi Yuan Yang

研究成果: 雜誌貢獻文章同行評審

16 引文 斯高帕斯(Scopus)

摘要

Zika virus (ZIKV) is a new and emerging virus that has caused outbreaks worldwide. The virus has been linked to congenital neurological malformations in neonates and Guillain–Barré syndrome in adults. Currently there are no effective vaccines available. As a result, there is a great need for ZIKV treatment. In this study, we developed single chain variable fragment (scFv) antibodies that target the ZIKV envelope protein using phage display technology. We first induced an immune response in white leghorn laying hens against the ZIKV envelope (E) protein. Chickens were immunized and polyclonal immunoglobulin yolk (IgY) antibodies were extracted from egg yolks. A high-level titer of anti-ZIKV_E IgY antibodies was detected using enzyme-linked immunosorbent assay (ELISA) after the third immunization. The titer persisted for at least 9 weeks. We constructed two antibody libraries that contained 5.3 × 106 and 4.5 × 106 transformants. After biopanning, an ELISA phage assay confirmed the enrichment of specific clones. We randomly selected 26 clones that expressed ZIKV scFv antibodies and classified them into two groups, short-linker and long-linker. Of these, four showed specific binding activities toward ZIKV_E proteins. These data suggest that the polyclonal and monoclonal scFv antibodies have the diagnostic or therapeutic potential for ZIKV.
原文英語
文章編號492
期刊International Journal of Molecular Sciences
21
發行號2
DOIs
出版狀態已發佈 - 1月 13 2020

ASJC Scopus subject areas

  • 催化
  • 分子生物學
  • 光譜
  • 電腦科學應用
  • 物理與理論化學
  • 有機化學
  • 無機化學

指紋

深入研究「Expression, purification, and characterization of anti-Zika virus envelope protein: Polyclonal and chicken-derived single chain variable fragment antibodies」主題。共同形成了獨特的指紋。

引用此