TY - JOUR
T1 - Experimental ocular toxocariasis in mice infected with long-term-maintained embryonated eggs of Toxocara canis
AU - Fan, Chia Kwung
AU - Lin, Yun Ho
AU - Hung, Chien Ching
AU - Su, Kua Eyre
N1 - Funding Information:
The authors are grateful to Taipei Medical University for its support of this research (grant no. TMU-86-Y05-B101). We also thank Mr. D.J. Kao and Mr. C.W. Liao for expert technical assistance, and the Taipei Municipal Institute for Animal Health is acknowledged for providing experimental stray dogs from which adult T. canis worms were collected.
PY - 2004/7
Y1 - 2004/7
N2 - Ocular injury to ICR mice from embryonated eggs of Toxocara canis, which had been maintained in 2% formalin for 14 months at 4°C, was evaluated by microscopic and pathologic assessments at 1, 2, 3, 5, 7, 28, 56, 84, 112, 140, 168, 196, 294 and 469 days post infection (DPI). On each date, three infected mice and two age-matched uninfected control mice were sacrificed; left eyeballs from infected mice were examined for larvae under a dissecting microscope while all right eyeballs were embedded in paraffin for histologic study. No larvae were observed in the left eyeball of any of the 42 infected mice, while pathologic changes were observed in right eyeballs. Pathologically, the predominant changes were retinal detachment, iridocyclitis, and pars planitis, followed by optic nerve papillitis and superficial infarcts, as observed in one of three infected mice per group at 56, 140, and 168 DPI, respectively. No infiltrating cells surrounding the larvae in the retina were observed in the infected mice. This is the first report that larvae hatched from T. canis embryonated eggs maintained for a long time can cause murine ocular toxocariasis. These results further extend our knowledge of the pathogenicity of T. canis embryonated eggs.
AB - Ocular injury to ICR mice from embryonated eggs of Toxocara canis, which had been maintained in 2% formalin for 14 months at 4°C, was evaluated by microscopic and pathologic assessments at 1, 2, 3, 5, 7, 28, 56, 84, 112, 140, 168, 196, 294 and 469 days post infection (DPI). On each date, three infected mice and two age-matched uninfected control mice were sacrificed; left eyeballs from infected mice were examined for larvae under a dissecting microscope while all right eyeballs were embedded in paraffin for histologic study. No larvae were observed in the left eyeball of any of the 42 infected mice, while pathologic changes were observed in right eyeballs. Pathologically, the predominant changes were retinal detachment, iridocyclitis, and pars planitis, followed by optic nerve papillitis and superficial infarcts, as observed in one of three infected mice per group at 56, 140, and 168 DPI, respectively. No infiltrating cells surrounding the larvae in the retina were observed in the infected mice. This is the first report that larvae hatched from T. canis embryonated eggs maintained for a long time can cause murine ocular toxocariasis. These results further extend our knowledge of the pathogenicity of T. canis embryonated eggs.
KW - Embryonated eggs
KW - ICR mice
KW - Ocular toxocariasis
KW - Toxocara canis
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U2 - 10.1016/s1607-551x(09)70164-0
DO - 10.1016/s1607-551x(09)70164-0
M3 - Article
AN - SCOPUS:4143147352
SN - 0257-5655
VL - 20
SP - 317
EP - 322
JO - Kaohsiung Journal of Medical Sciences
JF - Kaohsiung Journal of Medical Sciences
IS - 7
ER -