TY - JOUR
T1 - Exosomal proteomics in kidney disease
T2 - From technical approaches to clinical applications
AU - Brahmadhi, Ageng
AU - Chuang, Yung Kun
AU - Wang, San Yuan
AU - Kao, Chih Chin
AU - Tsai, I. Lin
N1 - Funding Information:
The authors acknowledge the academic and science graphic illustration service provided by the TMU Office of Research and Development. This work was financially supported by the Higher Education Sprout Project of the Ministry of Education (MOE) in Taiwan (DP2-110-21121-01-T-03-03).
Publisher Copyright:
© 2022 Taiwan Food and Drug Administration.
PY - 2022
Y1 - 2022
N2 - Exosomes are small extracellular vesicles (sEVs) secreted from cells and have a general diameter ranging from 30-150 nm. It was reported that exosomes have essential roles in intercellular communication and can be targeted as biomarkers of disease or as therapeutic agents. Among the different techniques used for exosome investigation, the mass spectrometry-based proteomics approach has accelerated the unraveling of the molecular composition of exosomes and has contributed to improved knowledge of molecular processes in various diseases. In this review, we focused on proteomics-based studies of exosomes and clinical applications in kidney diseases. A general introduction of exosomes, isolation and characterization techniques, and proteomics-based study workflows are included in this article. We also categorized applications in acute kidney injury, chronic kidney disease, renal transplantation, congenital kidney disease, and malignant kidney disorder to show the important findings from proteomics-based exosomal investigations.
AB - Exosomes are small extracellular vesicles (sEVs) secreted from cells and have a general diameter ranging from 30-150 nm. It was reported that exosomes have essential roles in intercellular communication and can be targeted as biomarkers of disease or as therapeutic agents. Among the different techniques used for exosome investigation, the mass spectrometry-based proteomics approach has accelerated the unraveling of the molecular composition of exosomes and has contributed to improved knowledge of molecular processes in various diseases. In this review, we focused on proteomics-based studies of exosomes and clinical applications in kidney diseases. A general introduction of exosomes, isolation and characterization techniques, and proteomics-based study workflows are included in this article. We also categorized applications in acute kidney injury, chronic kidney disease, renal transplantation, congenital kidney disease, and malignant kidney disorder to show the important findings from proteomics-based exosomal investigations.
KW - Exosome
KW - Extracellular vesicle
KW - Kidney-related diseases
KW - Mass spectrometry
KW - Proteomics
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U2 - 10.38212/2224-6614.3409
DO - 10.38212/2224-6614.3409
M3 - Review article
AN - SCOPUS:85132545239
SN - 1021-9498
VL - 30
SP - 202
EP - 222
JO - Journal of Food and Drug Analysis
JF - Journal of Food and Drug Analysis
IS - 2
ER -