The aim of this study is to assess the application of multiphoton auto fluorescence and second harmonic generation (SHG) microscopy for investigating corneal wound healing after high myopic (-10.OD) photorefractive keratectomy (PRK) procedures on the rabbit eyes. The effect of PRK on the morphology and distribution of keratocytes were investigated using multiphoton excited autofluorescence imaging, while the effect of PRK on the arrangement of collagen fibers was monitored by second-harmonic generation imaging. Without histological processing, multiphoton microscopy is able to characterize corneal damage and wound healing from PRK. Our results show that this technique has potential application in the clinical evaluation of corneal damage due to refractive surgery, and may be used to study the unwanted side effects of these procedures.
|名字||Progress in Biomedical Optics and Imaging - Proceedings of SPIE|
|其他||Ophthalmic Technologies XVIII|
|城市||San Jose, CA|
|期間||1/19/08 → 1/21/08|