TY - JOUR
T1 - Evaluation of the frequencies of chromosomal aberrations in a population exposed to prolonged low dose-rate 60Co γ-irradiation
AU - Hsieh, Wanhua A.
AU - Ni, Chyiyao
AU - Hwang, Jeng Jong
AU - Fang, Jye Siung
AU - Lin, Shuan Pei
AU - Lin, Yiping A.
AU - Huang, T. W.
AU - Chang, Wushou P.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2002
Y1 - 2002
N2 - Purpose: Chromosomal aberration analysis in peripheral blood lymphocytes was performed to evaluate late cytogenetic effects of long-term low dose-rate γ-irradiation exposure among students and residents exposed in radiocontaminated buildings. Materials and methods: Blood samples were taken from 1913 subjects (age 17.8±13.6, mean±SD) 5-8 years after their relocation from radioactive environments as well as from 176 non-exposed subjects (age 29.6±11.9) from the local community. Their lymphocytes were cultured for 48 h and metaphase spreads were prepared. A total of 208 900 metaphases were analysed for different types of chromosomal aberrations. Results: Relatively higher frequencies of translocations (2.1 × 10-3), rings (0.6 × 10-3) and dicentrics (0.6 × 10-3) were noted in the exposed population as compared with the non-exposed reference populations. Moreover, 356 (78.6%) of the 453 inversions were found on 14q11.2q32 in the exposed population. Among 392 well-demonstrated translocations, 167 (42.6%) and 175 (44.6%) occurred in chromosomes 7 and 14, respectively, while 139 (35.5%) occurred as t(7;14). In particular, the aberrations t(7;14)(p13;q11.2), t(7;14)(p15;q11.2) and t(7;14)(q36;q11.2) were the most prevalent, occurring with frequencies of 19 (13.7%), 20 (14.4%) and 27 (19.4%), respectively. In these, 3205 breakpoints were documented, with chromosomes 7, 9 and 14 shown to carry significantly higher frequencies of breakpoints than expected (χ2-test, p<0.0001). A further six hotspots were identified on 7p15 (57, 1.8%), 7q36 (42, 1.3%), 9q12 (244, 7.6%), 9q13 (86, 2.7%), 14q11.2 (509, 15.9%) and 14q32 (387, 12.1%) in the exposed population. Conclusion: In comparison with the unexposed population, we observed increased frequencies of various chromosomal aberrations in this human population with previous exposure to prolonged low dose-rate γ-radiation. Moreover, several hotspot breakpoints and inversions and translocations were observed on chromosomes 7 and 14.
AB - Purpose: Chromosomal aberration analysis in peripheral blood lymphocytes was performed to evaluate late cytogenetic effects of long-term low dose-rate γ-irradiation exposure among students and residents exposed in radiocontaminated buildings. Materials and methods: Blood samples were taken from 1913 subjects (age 17.8±13.6, mean±SD) 5-8 years after their relocation from radioactive environments as well as from 176 non-exposed subjects (age 29.6±11.9) from the local community. Their lymphocytes were cultured for 48 h and metaphase spreads were prepared. A total of 208 900 metaphases were analysed for different types of chromosomal aberrations. Results: Relatively higher frequencies of translocations (2.1 × 10-3), rings (0.6 × 10-3) and dicentrics (0.6 × 10-3) were noted in the exposed population as compared with the non-exposed reference populations. Moreover, 356 (78.6%) of the 453 inversions were found on 14q11.2q32 in the exposed population. Among 392 well-demonstrated translocations, 167 (42.6%) and 175 (44.6%) occurred in chromosomes 7 and 14, respectively, while 139 (35.5%) occurred as t(7;14). In particular, the aberrations t(7;14)(p13;q11.2), t(7;14)(p15;q11.2) and t(7;14)(q36;q11.2) were the most prevalent, occurring with frequencies of 19 (13.7%), 20 (14.4%) and 27 (19.4%), respectively. In these, 3205 breakpoints were documented, with chromosomes 7, 9 and 14 shown to carry significantly higher frequencies of breakpoints than expected (χ2-test, p<0.0001). A further six hotspots were identified on 7p15 (57, 1.8%), 7q36 (42, 1.3%), 9q12 (244, 7.6%), 9q13 (86, 2.7%), 14q11.2 (509, 15.9%) and 14q32 (387, 12.1%) in the exposed population. Conclusion: In comparison with the unexposed population, we observed increased frequencies of various chromosomal aberrations in this human population with previous exposure to prolonged low dose-rate γ-radiation. Moreover, several hotspot breakpoints and inversions and translocations were observed on chromosomes 7 and 14.
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U2 - 10.1080/09553000210132289
DO - 10.1080/09553000210132289
M3 - Article
C2 - 12079542
AN - SCOPUS:0036295427
SN - 0955-3002
VL - 78
SP - 625
EP - 633
JO - International Journal of Radiation Biology
JF - International Journal of Radiation Biology
IS - 7
ER -