TY - JOUR
T1 - Evaluation of porous bacterial cellulose produced from foam templating with different additives and its application in 3D cell culture
AU - Lin, Shin Ping
AU - Singajaya, Stephanie
AU - Lo, Tsui Yun
AU - Santoso, Shella Permatasari
AU - Hsu, Hsien Yi
AU - Cheng, Kuan Chen
N1 - Funding Information:
This work was financially supported by Taipei Medical University, National Taiwan University, and the Ministry of Science and Technology, Taiwan, through research grants with contract nos. MOST109-2628-E-002-007-MY3 and MOST110-2221-E-038-003-MY3. The authors acknowledge the academic and science graphic illustration services provided by the TMU Office of Research and Development.
Funding Information:
This work was financially supported by Taipei Medical University , National Taiwan University , and the Ministry of Science and Technology, Taiwan , through research grants with contract nos. MOST109-2628-E-002-007-MY3 and MOST110-2221-E-038-003-MY3 . The authors acknowledge the academic and science graphic illustration services provided by the TMU Office of Research and Development.
Publisher Copyright:
© 2023
PY - 2023/4
Y1 - 2023/4
N2 - Bacterial cellulose (BC) is used in biomedical applications due to its unique material properties such as mechanical strength with a high water-absorbing capacity and biocompatibility. Nevertheless, native BC lacks porosity control which is crucial for regenerative medicine. Hence, developing a simple technique to change the pore sizes of BC has become an important issue. This study combined current foaming BC (FBC) production with incorporation of different additives (avicel, carboxymethylcellulose, and chitosan) to form novel porous additive-altered FBC. Results demonstrated that the FBC samples provided greater reswelling rates (91.57 % ~ 93.67 %) compared to BC samples (44.52 % ~ 67.5 %). Moreover, the FBC samples also showed excellent cell adhesion and proliferation abilities for NIH-3T3 cells. Lastly, FBC allowed cells to penetrate to deep layers for cell adhesion due to its porous structure, providing a competitive scaffold for 3D cell culture in tissue engineering.
AB - Bacterial cellulose (BC) is used in biomedical applications due to its unique material properties such as mechanical strength with a high water-absorbing capacity and biocompatibility. Nevertheless, native BC lacks porosity control which is crucial for regenerative medicine. Hence, developing a simple technique to change the pore sizes of BC has become an important issue. This study combined current foaming BC (FBC) production with incorporation of different additives (avicel, carboxymethylcellulose, and chitosan) to form novel porous additive-altered FBC. Results demonstrated that the FBC samples provided greater reswelling rates (91.57 % ~ 93.67 %) compared to BC samples (44.52 % ~ 67.5 %). Moreover, the FBC samples also showed excellent cell adhesion and proliferation abilities for NIH-3T3 cells. Lastly, FBC allowed cells to penetrate to deep layers for cell adhesion due to its porous structure, providing a competitive scaffold for 3D cell culture in tissue engineering.
KW - 3D cell culture
KW - Bacterial cellulose
KW - Foam templating
KW - Tissue scaffold
UR - http://www.scopus.com/inward/record.url?scp=85148343020&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85148343020&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2023.123680
DO - 10.1016/j.ijbiomac.2023.123680
M3 - Article
C2 - 36801225
AN - SCOPUS:85148343020
SN - 0141-8130
VL - 234
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
M1 - 123680
ER -