Evaluation of polymerase chain reaction-restriction enzyme analysis of mycobacteria cultured in BACTEC 12B bottles

Tsi Shu Huang, Yung Ching Liu, Wen Kuei Huang, Chii Shiang Chen, Hui Zin Tu, Deh Lin Cheng

研究成果: 雜誌貢獻文章同行評審

1 引文 斯高帕斯(Scopus)

摘要

Polymerase chain reaction with restriction enzyme analysis (PRA) was first tested on 15 reference strains and 50 subcultured clinical isolates of mycobacteria according to the reference algorithm by Telenti et al [1]. Next, we evaluated the application of this method to 108 isolates from liquid media (BACTEC 12B). Of them, 15 M. tuberculosis complex and 81 mycobacteria other than tuberculosis (MOTT) had comparable results with both PRA and the BACTEC 460 TB systems. However, seven M. tuberculosis complex and three potentially pathogenic MOTT were identified by PRA rather than the BACTEC TB system. PRA seems to be an efficient method for the identification of mycobacteria to the species level and a good aid to detect potentially pathogenic mycobacteria, especially in mixed mycobacterial cultures.

原文英語
頁(從 - 到)530-535
頁數6
期刊Journal of the Formosan Medical Association = Taiwan yi zhi
95
發行號7
出版狀態已發佈 - 7月 1996
對外發佈

ASJC Scopus subject areas

  • 醫藥 (全部)

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