Evaluation of Pm2.5 Influence on Human Lung Cancer Cells Using a Microfluidic Platform

Uyen Thi Nhat Nguyen, Han Yun Hsieh, Tzu Yun Chin, Guani Wu, Yen Po Lin, Ching Yi Lee, Yi Chiung Hsu, Yu Jui Fan

研究成果: 雜誌貢獻文章同行評審

摘要

In this study, we developed a microfluidic device that is able to monitor cell biology under continuous PM2.5 treatment. The effects of PM2.5 on human alveolar basal epithelial cells, A549 cells, and uncovered several significant findings were investigated. The results showed that PM2.5 exposure did not lead to a notable decrease in cell viability, indicating that PM2.5 did not cause cellular injury or death. However, the study found that PM2.5 exposure increased the invasion and migration abilities of A549 cells, suggesting that PM2.5 might promote cell invasiveness. Results of RNA sequencing revealed 423 genes that displayed significant differential expression in response to PM2.5 exposure, with a particular focus on pathways associated with the generation of reactive oxygen species (ROS) and mitochondrial dysfunction. Real-time detection demonstrated an increase in ROS production in A549 cells after exposure to PM2.5. JC1 assay, which indicated a loss of mitochondrial membrane potential (ΔΨm) in A549 cells exposed to PM2.5. The disruption of mitochondrial membrane potential further supports the detrimental effects of PM2.5 on A549 cells. These findings highlight several adverse effects of PM2.5 on A549 cells, including enhanced invasion and migration capabilities, altered gene expression related to ROS pathways, increased ROS production and disruption of mitochondrial membrane potential. These findings contribute to our understanding of the potential mechanisms through which PM2.5 can impact cellular function and health.
原文英語
頁(從 - 到)1117-1128
頁數12
期刊International Journal of Medical Sciences
21
發行號6
DOIs
出版狀態已發佈 - 2024

ASJC Scopus subject areas

  • 一般醫學

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