Evaluation of expanded bed adsorption chromatography for extraction of prothrombin complex from Cohn Supernatant I

Karl B. McCann; Peter T. Gomme; John Wu; Joseph Bertolini

doi:10.1016/j.biologicals.2008.01.002

Evaluation of expanded bed adsorption chromatography for extraction of prothrombin complex from Cohn Supernatant I

Karl B. McCann, Peter T. Gomme, John Wu, Joseph Bertolini

研究成果: 雜誌貢獻 › 文章 › 同行評審

4 引文斯高帕斯（Scopus）

摘要

This study evaluated the feasibility of substituting expanded bed adsorption (EBA) chromatography for an existing chromatographic purification process for the isolation of prothrombin complex concentrate (PCC) from Cohn Supernatant I. The EBA chromatography (Streamline) resins were compared to the current DEAE-cellulose resin for the extraction of PCC from Cohn SNI. EBA chromatography resins efficiently bound PCC from Cohn SNI at a significantly higher flow rate of up to 300 cm/h compared to 30 cm/h for the current DEAE-cellulose process. Composition and yield of the recovered PCC reflected the elution conditions used. The results indicate that EBA chromatography could be used to efficiently produce PCC comparable to existing products.

原文	英語
頁（從 - 到）	227-233
頁數	7
期刊	Biologicals
卷	36
發行號	4
DOIs	https://doi.org/10.1016/j.biologicals.2008.01.002
出版狀態	已發佈 - 7月 1 2008
對外發佈	是

ASJC Scopus subject areas

免疫學
微生物學
傳染性疾病

UN SDG

此研究成果有助於以下永續發展目標

存取文件

10.1016/j.biologicals.2008.01.002

其它文件與鏈接

Link to publication in Scopus

引用此

@article{74d1882ce8e04c3ea1fdd7581408ee58,

title = "Evaluation of expanded bed adsorption chromatography for extraction of prothrombin complex from Cohn Supernatant I",

abstract = "This study evaluated the feasibility of substituting expanded bed adsorption (EBA) chromatography for an existing chromatographic purification process for the isolation of prothrombin complex concentrate (PCC) from Cohn Supernatant I. The EBA chromatography (Streamline) resins were compared to the current DEAE-cellulose resin for the extraction of PCC from Cohn SNI. EBA chromatography resins efficiently bound PCC from Cohn SNI at a significantly higher flow rate of up to 300 cm/h compared to 30 cm/h for the current DEAE-cellulose process. Composition and yield of the recovered PCC reflected the elution conditions used. The results indicate that EBA chromatography could be used to efficiently produce PCC comparable to existing products.",

keywords = "Cohn Supernatant I, Expanded bed adsorption, Plasma, Prothrombin complex concentrate",

author = "McCann, {Karl B.} and Gomme, {Peter T.} and John Wu and Joseph Bertolini",

year = "2008",

month = jul,

day = "1",

doi = "10.1016/j.biologicals.2008.01.002",

language = "English",

volume = "36",

pages = "227--233",

journal = "Biologicals",

issn = "1045-1056",

publisher = "Academic Press Inc.",

number = "4",

}

TY - JOUR

T1 - Evaluation of expanded bed adsorption chromatography for extraction of prothrombin complex from Cohn Supernatant I

AU - McCann, Karl B.

AU - Gomme, Peter T.

AU - Wu, John

AU - Bertolini, Joseph

PY - 2008/7/1

Y1 - 2008/7/1

N2 - This study evaluated the feasibility of substituting expanded bed adsorption (EBA) chromatography for an existing chromatographic purification process for the isolation of prothrombin complex concentrate (PCC) from Cohn Supernatant I. The EBA chromatography (Streamline) resins were compared to the current DEAE-cellulose resin for the extraction of PCC from Cohn SNI. EBA chromatography resins efficiently bound PCC from Cohn SNI at a significantly higher flow rate of up to 300 cm/h compared to 30 cm/h for the current DEAE-cellulose process. Composition and yield of the recovered PCC reflected the elution conditions used. The results indicate that EBA chromatography could be used to efficiently produce PCC comparable to existing products.

AB - This study evaluated the feasibility of substituting expanded bed adsorption (EBA) chromatography for an existing chromatographic purification process for the isolation of prothrombin complex concentrate (PCC) from Cohn Supernatant I. The EBA chromatography (Streamline) resins were compared to the current DEAE-cellulose resin for the extraction of PCC from Cohn SNI. EBA chromatography resins efficiently bound PCC from Cohn SNI at a significantly higher flow rate of up to 300 cm/h compared to 30 cm/h for the current DEAE-cellulose process. Composition and yield of the recovered PCC reflected the elution conditions used. The results indicate that EBA chromatography could be used to efficiently produce PCC comparable to existing products.

KW - Cohn Supernatant I

KW - Expanded bed adsorption

KW - Plasma

KW - Prothrombin complex concentrate

UR - http://www.scopus.com/inward/record.url?scp=45949086931&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=45949086931&partnerID=8YFLogxK

U2 - 10.1016/j.biologicals.2008.01.002

DO - 10.1016/j.biologicals.2008.01.002

M3 - Article

C2 - 18329287

AN - SCOPUS:45949086931

SN - 1045-1056

VL - 36

SP - 227

EP - 233

JO - Biologicals

JF - Biologicals

IS - 4

ER -

Evaluation of expanded bed adsorption chromatography for extraction of prothrombin complex from Cohn Supernatant I

摘要

ASJC Scopus subject areas

UN SDG

存取文件

其它文件與鏈接

指紋

引用此