TY - JOUR
T1 - Epidermal growth factor enhances transcription of human arachidonate 12-lipoxygenase in A431 cells
AU - Liu, Yi Wen
AU - Chen, Ben Kuen
AU - Chen, Ching Jiunn
AU - Arakawa, Toshiya
AU - Yoshimoto, Tanihiro
AU - Yamamoto, Shozo
AU - Chang, Wen Chang
N1 - Funding Information:
We thanks Dr. Kun-yen Huang for critical review of this manuscript and Y.-L. Chang for secretarial assistance. This work was supported in part by Grant NSC84-2331-B006-064 from the National Science Council of the Republic of China (W.C.C.) and by grants-in-aid from the Ministry of Education, Science and culture, the Japanese Foundation of Metabolism and Disease, the Japan Foundation for Applied Enzymology, the Ono Medical Research Foundation and the Ciba-Geigy Foundation for the Promotion of Science (T.Y., Y.S.).
PY - 1997/1/7
Y1 - 1997/1/7
N2 - Epidermal growth factor (EGF), determined by immunoprecipitation and Western blot analysis, increased both enzyme activity and protein level of 12-lipoxygenase in the solubilized microsomes of human epidermoid carcinoma A431 cells, respectively. The EGF-induced expression of 12-lipoxygenase mRNA was inhibited by transcription inhibitors such as actinomycin D and 5,6-dichlorobenzimidazole riboside. Promoters of different lengths for human 12-lipoxygenase gene were used to prepare the luciferase fusion vectors. These construct plasmids were transiently transfected into A431 cells, and the induction of luciferase expression by EGF was examined. A 4- to 6-fold increase in luciferase reporter activity stimulated by EGF for 18 h treatment was observed in plasmids with the 5'-flanking region length of -951 bp and that of -224 bp upstream from translation starting site. The time-dependent induction of luciferase activity by EGF paralleled the EGF-induced enzyme activity and expression of 12-lipoxygenase protein. Taken together, the results of this study indicate that EGF enhanced the transcription of the human 12-lipoxygenase gene, resulting in an increase in the amount and activity of 12-lipoxygenase.
AB - Epidermal growth factor (EGF), determined by immunoprecipitation and Western blot analysis, increased both enzyme activity and protein level of 12-lipoxygenase in the solubilized microsomes of human epidermoid carcinoma A431 cells, respectively. The EGF-induced expression of 12-lipoxygenase mRNA was inhibited by transcription inhibitors such as actinomycin D and 5,6-dichlorobenzimidazole riboside. Promoters of different lengths for human 12-lipoxygenase gene were used to prepare the luciferase fusion vectors. These construct plasmids were transiently transfected into A431 cells, and the induction of luciferase expression by EGF was examined. A 4- to 6-fold increase in luciferase reporter activity stimulated by EGF for 18 h treatment was observed in plasmids with the 5'-flanking region length of -951 bp and that of -224 bp upstream from translation starting site. The time-dependent induction of luciferase activity by EGF paralleled the EGF-induced enzyme activity and expression of 12-lipoxygenase protein. Taken together, the results of this study indicate that EGF enhanced the transcription of the human 12-lipoxygenase gene, resulting in an increase in the amount and activity of 12-lipoxygenase.
KW - 12-Lipoxygenase
KW - Epidermal growth factor
KW - Human epidermoid carcinoma A431 cell
KW - Transcription
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U2 - 10.1016/S0005-2760(96)00128-2
DO - 10.1016/S0005-2760(96)00128-2
M3 - Article
C2 - 9022753
AN - SCOPUS:0031556931
SN - 1388-1981
VL - 1344
SP - 38
EP - 46
JO - BBA - Specialised Section On Lipids and Related Subjects
JF - BBA - Specialised Section On Lipids and Related Subjects
IS - 1
ER -