Elucidating the signaling mechanism of an epithelial tight-junction opening induced by chitosan

Li Wen Hsu, Pei Ling Lee, Chiung Tong Chen, Fwu Long Mi, Jyuhn Huarng Juang, Shiaw Min Hwang, Yi Cheng Ho, Hsing Wen Sung

研究成果: 雜誌貢獻文章同行評審

72 引文 斯高帕斯(Scopus)


Chitosan (CS) and its derivatives have been investigated as paracellular permeation enhancers for facilitating the oral bioavailability of hydrophilic macromolecules. As is well known, CS can transiently open the tight junctions (TJs) between epithelial cells, thus enhancing the paracellular permeability. However, the signaling mechanism that is related to the effect of CS on TJs remains unclear. Therefore, this study elucidates the potential transduction cascade of TJ opening in Caco-2 cell monolayers subsequent to CS exposure. Experimental results indicate that activation of integrin receptors on cell membranes significantly contributes to CS-mediated TJ disruption, initiating the cascade of TJ opening. Additionally, treatment of Caco-2 cell monolayers with CS leads to the clustering of integrins along the cell border, phosphorylation of FAK and Src tyrosine kinases, and results in the regulation of TJ permeability via the redistribution of TJ protein CLDN4 from the cell membrane to the cytosol. Elucidating the signaling mechanism of CS-induced TJ opening in intestinal cells significantly contributes to efforts to use CS and its derivatives as paracellular permeation enhancers.

頁(從 - 到)6254-6263
出版狀態已發佈 - 9月 2012

ASJC Scopus subject areas

  • 生物工程
  • 陶瓷和複合材料
  • 生物物理學
  • 生物材料
  • 材料力學


深入研究「Elucidating the signaling mechanism of an epithelial tight-junction opening induced by chitosan」主題。共同形成了獨特的指紋。