TY - JOUR
T1 - Electroelution of DNA and protein from polyacrylamide and agarose gels.
AU - Chang, T.
AU - Tsai, I. H.
AU - Chang, W. C.
PY - 1987/10/1
Y1 - 1987/10/1
N2 - An electroelution method is described for the recovery of DNA and protein from agarose or polyacrylamide gels. The samples to be electroeluted are compartmentalized in a modified microcentrifuge tube fitted with dialysis membranes. This procedure is simple, rapid, inexpensive and efficient. Within 30 min to 2 hrs, the recovery of the sample is nearly quantitative. DNA fragments recovered can be directly subjected to DNA sequence analysis or enzymatic reactions after ethanol precipitation. Proteins can also be recovered after separation by acrylamide gel in the presence or absence of detergents and be ready for further analysis.
AB - An electroelution method is described for the recovery of DNA and protein from agarose or polyacrylamide gels. The samples to be electroeluted are compartmentalized in a modified microcentrifuge tube fitted with dialysis membranes. This procedure is simple, rapid, inexpensive and efficient. Within 30 min to 2 hrs, the recovery of the sample is nearly quantitative. DNA fragments recovered can be directly subjected to DNA sequence analysis or enzymatic reactions after ethanol precipitation. Proteins can also be recovered after separation by acrylamide gel in the presence or absence of detergents and be ready for further analysis.
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M3 - Article
C2 - 3435534
AN - SCOPUS:0023431322
SN - 0158-5231
VL - 15
SP - 687
EP - 691
JO - Biochemistry International
JF - Biochemistry International
IS - 4
ER -