TY - JOUR
T1 - Effects of propofol on proliferation and anti-apoptosis of neuroblastoma SH-SY5Y cell line
T2 - New insights into neuroprotection
AU - Wu, Gong-Jhe
AU - Chen, Wu Fu
AU - Hung, Han Chun
AU - Jean, Yen Hsuan
AU - Sung, Chun Sung
AU - Chakraborty, Chiranjib
AU - Lee, Hsin Pai
AU - Chen, Nan Fu
AU - Wen, Zhi Hong
PY - 2011/4/12
Y1 - 2011/4/12
N2 - Recently, it has been suggested that anesthetic agents may have neuroprotective potency. The notion that anesthetic agents can offer neuroprotection remains controversial. Propofol, which is a short-acting intravenous anesthetic agent, may have potential as a neuroprotective agent. In this study, we tried to determine whether propofol affected the viability of human neuroblastoma SH-SY5Y cells by using the MTT assay. Surprisingly, our results showed that propofol at a dose of 1-10 μM could improve cell proliferation. However, at higher doses (200 μM), propofol appears to be cytotoxic. On the other hand, propofol could up-regulate the expression of key proteins involved in neuroprotection including B-cell lymphoma 2 at a dose range of 1-10 μM, activation of phospho-serine/threonine protein kinase at a dose range of 0.5-10 μM, and activation of phospho-extracellular signal-regulated kinases at a dose range of 5-10 μM. Similarly, we demonstrate that propofol (10 μM) could elevate protein levels of heat shock protein 90 and heat shock protein 70. Therefore, we choose to utilize a 10 μM concentration of propofol to assess neuroprotective activities in our studies. In the following experiments, we used dynorphin A to generate cytotoxic effects on SH-SY5Y cells. Our data indicate that propofol (10 μM) could inhibit the cytotoxicity in SH-SY5Y cells induced by dynorphin A. Furthermore, propofol (10 μM) could decrease the expression of the p-P38 protein as well. These data together suggest that propofol may have the potential to act as a neuroprotective agent against various neurologic diseases. However, further delineation of the precise neuroprotective effects of propofol will need to be examined.
AB - Recently, it has been suggested that anesthetic agents may have neuroprotective potency. The notion that anesthetic agents can offer neuroprotection remains controversial. Propofol, which is a short-acting intravenous anesthetic agent, may have potential as a neuroprotective agent. In this study, we tried to determine whether propofol affected the viability of human neuroblastoma SH-SY5Y cells by using the MTT assay. Surprisingly, our results showed that propofol at a dose of 1-10 μM could improve cell proliferation. However, at higher doses (200 μM), propofol appears to be cytotoxic. On the other hand, propofol could up-regulate the expression of key proteins involved in neuroprotection including B-cell lymphoma 2 at a dose range of 1-10 μM, activation of phospho-serine/threonine protein kinase at a dose range of 0.5-10 μM, and activation of phospho-extracellular signal-regulated kinases at a dose range of 5-10 μM. Similarly, we demonstrate that propofol (10 μM) could elevate protein levels of heat shock protein 90 and heat shock protein 70. Therefore, we choose to utilize a 10 μM concentration of propofol to assess neuroprotective activities in our studies. In the following experiments, we used dynorphin A to generate cytotoxic effects on SH-SY5Y cells. Our data indicate that propofol (10 μM) could inhibit the cytotoxicity in SH-SY5Y cells induced by dynorphin A. Furthermore, propofol (10 μM) could decrease the expression of the p-P38 protein as well. These data together suggest that propofol may have the potential to act as a neuroprotective agent against various neurologic diseases. However, further delineation of the precise neuroprotective effects of propofol will need to be examined.
KW - Dynorphin A
KW - Heat shock protein
KW - Human neuroblastoma cell
KW - Neuroprotection
KW - Proliferation
KW - Propofol
KW - Survival (antiapoptotic) signaling proteins
UR - http://www.scopus.com/inward/record.url?scp=79953027222&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79953027222&partnerID=8YFLogxK
U2 - 10.1016/j.brainres.2011.02.004
DO - 10.1016/j.brainres.2011.02.004
M3 - Article
C2 - 21315692
AN - SCOPUS:79953027222
SN - 0006-8993
VL - 1384
SP - 42
EP - 50
JO - Brain Research
JF - Brain Research
ER -