TY - JOUR
T1 - Effects of epidermal growth factor and its signal transduction inhibitors on apoptosis in human colorectal cancer cells
AU - Chao, J. C J
AU - Peng, Wen L.
AU - Chen, Sheng-Hsuan
PY - 2004/2/15
Y1 - 2004/2/15
N2 - Aim: The study investigated if EGF signaling inhibitors, EGF antibody and tyrphostin 51 (a tyrosine kinase inhibitor), mediated the action of EGF on apoptosis and the expression of EGF receptors and p21 (a cyclin-dependent kinase inhibitor) of human colorectal cancer cells. Methods: Human colorectal adenocarcinoma cells (SW480) were incubated with 0.6 mL/L dimethyl sulfoxide (DMSO, the control group), 225 ng/mL (37.5 nmol/L) EGF in 0.6 mL/L DMSO, 225 ng/mL EGF+2.5 μg/mL (17 nmol/L) EGF antibody in 0.6 mL/L DMSO, or 225 ng/mL EGF+215 ng/mL (0.8 μmol/L) tyrphostin 51 in 0.6 mL/L DMSO. Results: After 48 h incubation, the levels of EGF in medium significantly increased (P
AB - Aim: The study investigated if EGF signaling inhibitors, EGF antibody and tyrphostin 51 (a tyrosine kinase inhibitor), mediated the action of EGF on apoptosis and the expression of EGF receptors and p21 (a cyclin-dependent kinase inhibitor) of human colorectal cancer cells. Methods: Human colorectal adenocarcinoma cells (SW480) were incubated with 0.6 mL/L dimethyl sulfoxide (DMSO, the control group), 225 ng/mL (37.5 nmol/L) EGF in 0.6 mL/L DMSO, 225 ng/mL EGF+2.5 μg/mL (17 nmol/L) EGF antibody in 0.6 mL/L DMSO, or 225 ng/mL EGF+215 ng/mL (0.8 μmol/L) tyrphostin 51 in 0.6 mL/L DMSO. Results: After 48 h incubation, the levels of EGF in medium significantly increased (P
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U2 - 10.3748/wjg.v10.i4.540
DO - 10.3748/wjg.v10.i4.540
M3 - Article
C2 - 14966913
AN - SCOPUS:1542345022
SN - 1007-9327
VL - 10
SP - 540
EP - 544
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
IS - 4
ER -
