Effects of Arginine Supplementation on Exogenous Advanced Glycation End Product-induced Renal Inflammatory Mediator Expression in Rats

Background: This study investigated the effects of dietary Arg supplementation on renal inflammatory mediator expression and oxidative damage in rats with exogenous advanced glycation end product (AGE) administration. Methods: There were two groups of rats: the CA group was fed a common diet and given a tail vein injection of AGE-bovine serum albumin (BSA); and the AA group was fed the Arg-supplemented diet and injected with AGE-BSA. Arg provided 2% of the total energy. The tail vein injection and diets for the respective groups were given for 10 days. After that, all rats were sacrificed, and blood and kidneys were harvested for further analysis. Results: Blood adhesion molecule expression and NO levels were higher in the AA group. Also, the kidney nitrotyrosine concentration, phospho-nuclear factor-κB p65 and inducible NO synthase protein expression was higher in the AA group than in the CA group. The finding of immunohistochemical staining was consistent with the results that the AA group had higher receptor of AGE expression in the kidneys. Conclusion: Supplemental dietary Arg may have adverse effect in AGE-induced kidney inflammatory response and oxidative damage in rats.