Effects of Activin in Embryoid Bodies Expressing Fibroblast Growth Factor 5

Yasumasa Shirouzu, Goichi Yanai, Kai Chiang Yang, Shoichiro Sumi

研究成果: 雜誌貢獻文章同行評審

2 引文 斯高帕斯(Scopus)


Nodal/activin signaling is indispensable for embryonic development. We examined what activin does to the embryoid bodies (EBs) produced from mouse embryonic stem cells (mESCs) expressing an epiblast marker. The EBs were produced by culturing mESCs by the hanging drop method for 24 hours. The resulting EBs were transferred onto gelatin-coated dishes and allowed to further differentiate. The 24-hour EBs showed a stronger expression of fibroblast growth factor (FGF)5 and Brachyury (specific to the epiblast) in comparison with mESCs. Treating the transferred EBs with activin A maintained transcript levels of FGF5 and Oct4, while inhibiting definitive endoderm differentiation. The activin A treatment reversed the endoderm differentiation induced by retinoic acid (RA), while the inhibition of nodal/activin signaling promoted RA-induced endoderm differentiation. Inhibition of nodal/activin signaling in EBs, including epiblast-like cells, promotes differentiation into the endoderm, facilitating the transition from the pluripotent state to specification of the endoderm.
頁(從 - 到)171-186
期刊Cellular Reprogramming
出版狀態已發佈 - 6月 1 2016

ASJC Scopus subject areas

  • 生物技術
  • 發展生物學
  • 細胞生物學


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