Effect of endothelin receptor antagonists on gap junction channels in post-infarcted rats

Tsung-Ming Lee, Chien-Chang Chen, Nen-Chung Chang

研究成果: 雜誌貢獻文章同行評審


Myocardial infarction leads to derangements in cellular electrical stability and the generation of lethal arrhythmias. Endothelin (ET) receptor blockers (ERAs) have been shown to contribute to the antiarrhythmic effect. We investigated whether ERAs exert antiarrhythmic effects through increased expression of connexin43 (Cx43) after infarction. Twenty-four hours after ligation of the left anterior descending artery, male Wistar rats received either vehicle, ABT-627 (selective ETA receptor antagonist), bosentan (nonselective ETA/ETB receptor antagonist), or hydralazine for 4 weeks. Measurement of myocardial ET-1 levels at the border zone revealed a significant increase in vehicle-treated rats compared with sham-operated rats, consistent with increased ET-1 levels after infarction. Immunoblotting revealed that at the border zone the vehicle group showed a marked reduction in the amount of phosphorylated Cx43, whereas either ABT-627- or bosentan-treated groups showed only a slight reduction compared with sham. Immunohistochemistry and real-time RT-PCR confirmed that the Cx43 attenuation was prevented by ERA administration. Arrhythmic scores during programmed stimulation in ERA-treated rats were significantly lower than those treated with vehicle. The effects of ERAs on Cx43 and arrhythmais were dissociated from their blood pressure-lowering effect because ERAs and hydralazine reduced arterial pressure similarly. Our data indicate that the ET system plays an important role in the attenuated expression of Cx43 after infarction. Chronic inhibition of either ETA or ETA/ETB receptors restores the decreased expression of phosphorylated Cx43 and improves electrical instability.
頁(從 - 到)1130.16
期刊FASEB Journal
出版狀態已發佈 - 4月 1 2008


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