Dose-dependent effect of Lactobacillus rhamnosus on quantitative reduction of faecal rotavirus shedding in children

Shiuh Bin Fang, Hung Chang Lee, Jen Jan Hu, Shao Yi Hou, Hsuan Liang Liu, Hsu Wei Fang

研究成果: 雜誌貢獻文章同行評審

78 引文 斯高帕斯(Scopus)

摘要

Beneficial effects of probiotics in acute infectious diarrhoea in children are mainly seen in watery diarrhoea and viral gastroenteritis. Lactobacillus rhamnosus, one the most extensively studied probiotic strains, is effective in shortening courses of acute diarrhoea in children. However, the dose-dependent effect of Lactobacillus upon quantification of faecal rotavirus shedding in humans remains little known. Thus, an open-label randomized trial in 23 children with acute rotaviral gastroenteritis was undertaken by randomly allocating patients to receive one of the three regimens for 3 days: daily. Lactobacillus rhamnosus 35 (Lcr35) with 0 CFU/day to six patients in the control group, 2 × 10. 8 CFU/day to nine patients in the low-dose group, and 6 × 10. 8 CFU/day to eight patients in the high-dose group. Faecal samples were collected before and after the 3-day regimen for measurements of rotavirus concentrations by ELISA. There was no statistically significant change in faecal rotavirus concentrations in either the control group (119.2 × 10. 5 particles/ml vs. 23.7 × 10. 5 particles/ml p = 0.075) or the low-dose group (36.1 × 10. 5 particles/ml vs. 73.5 × 10. 5 particles/ml p = 0.859). However, the high-dose group had a significant reduction of faecal rotavirus concentration (64.2 × 10. 5 particles/ml vs. 9.0 × 10. 5 particles/ml p = 0.012). Without any exception, the faecal rotavirus concentrations of all eight patients in the high-dose Lcr35 group declined by 86% after 3 days when compared with those before Lcr35 administration. In conclusion, this is the first report to provide quantitative evidence of the dose-dependent effect of Lactobacillus rhamnosus, a minimal effective dose of 6 × 10. 8 CFU for 3 days, upon the faecal rotavirus shedding in paediatric patients.
原文英語
文章編號fmp001
頁(從 - 到)297-301
頁數5
期刊Journal of Tropical Pediatrics
55
發行號5
DOIs
出版狀態已發佈 - 2月 8 2009
對外發佈

ASJC Scopus subject areas

  • 兒科、圍產兒和兒童健康
  • 傳染性疾病

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