DNA looping alters local DNA conformation during transcription

Hai Young Wu; Leroy F. Liu

doi:10.1016/0022-2836(91)90658-S

DNA looping alters local DNA conformation during transcription

Hai Young Wu, Leroy F. Liu

研究成果: 雜誌貢獻 › 文章 › 同行評審

11 引文斯高帕斯（Scopus）

摘要

The effect of protein-mediated DNA looping on local DNA conformation during active transcription was studied using the lac repressor-operator system. Our results suggest that lac repressor-mediated DNA looping within a plasmid DNA molecule containing two lac repressor binding sequences in vivo effectively separates plasmid DNA into two topological domains. Supercoils generated by transcription within each topological domain can be rapidly removed by DNA topoisomerase I.

原文	英語
頁（從 - 到）	615-622
頁數	8
期刊	Journal of Molecular Biology
卷	219
發行號	4
DOIs	https://doi.org/10.1016/0022-2836(91)90658-S
出版狀態	已發佈 - 6月 20 1991
對外發佈	是

ASJC Scopus subject areas

結構生物學
分子生物學

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10.1016/0022-2836(91)90658-S

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abstract = "The effect of protein-mediated DNA looping on local DNA conformation during active transcription was studied using the lac repressor-operator system. Our results suggest that lac repressor-mediated DNA looping within a plasmid DNA molecule containing two lac repressor binding sequences in vivo effectively separates plasmid DNA into two topological domains. Supercoils generated by transcription within each topological domain can be rapidly removed by DNA topoisomerase I.",

keywords = "DNA looping, DNA supercoiling, DNA topoisomerases, RNA transcription, lac repressor",

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AU - Liu, Leroy F.

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AB - The effect of protein-mediated DNA looping on local DNA conformation during active transcription was studied using the lac repressor-operator system. Our results suggest that lac repressor-mediated DNA looping within a plasmid DNA molecule containing two lac repressor binding sequences in vivo effectively separates plasmid DNA into two topological domains. Supercoils generated by transcription within each topological domain can be rapidly removed by DNA topoisomerase I.

KW - DNA looping

KW - DNA supercoiling

KW - DNA topoisomerases

KW - RNA transcription

KW - lac repressor

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DNA looping alters local DNA conformation during transcription

摘要

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