@article{d4aa66557ab147d587333c08c6f36f24,
title = "Direct coating of culture medium from cells secreting classical swine fever virus E2 antigen on ELISA plates for detection of E2-specific antibodies",
abstract = "The envelope glycoprotein E2 of classical swine fever virus (CSFV) is widely used as a marker for measuring vaccine efficacy and antibody titer. The glycosylation profile of E2 may affect the immunogenicity of the vaccine and the timing of re-vaccination. In this study, a human embryonic kidney cell line was used to secrete fully-glycosylated CSFV E2, which was then coated onto ELISA plates without purification or adjustment.The resulting E2-secreting medium-direct-coating (E2-mDc) ELISA was successfully used to measure anti-E2 antibody titers in vaccinated and field pig sera samples. Compared with a virus neutralization test (as standard), the E2-mDc ELISA was found to be more accurate (90%) than a commercial CSFV antibody diagnostic kit (62%). In conclusion, the mammalian cell-secreted antigen can provide cheap, accurate and effective assays for vaccine efficacy and disease diagnoses.",
keywords = "Classical swine fever virus, E2, Glycosylation, Mammalian expression, Medium-direct-coating ELISA",
author = "Cheng, {Ta Chun} and Pan, {Chu Hsiang} and Chen, {Chien Shu} and Chuang, {Kuo Hsiang} and Chuang, {Chih Hung} and Huang, {Chien Chaio} and Chu, {Yu Yi} and Yang, {Ya Chun} and Chu, {Pei Yu} and Kao, {Chien Han} and Hsieh, {Yuan Chin} and Cheng, {Tian Lu}",
note = "Funding Information: This work was supported by grants from the National Research Program for Biopharmaceuticals, Ministry of Science and Technology, Taiwan ( MOST 103-2325-B-037-007 , NSC 102-2320-B-038-043-MY2 ), the Ministry of Health and Welfare, Taiwan ( MOHW103-TD-B-111-05 ), the National Health Research Institutes , Taiwan ( NHRI-EX103-10238SC ), 103NSYSU-KMU Joint Research Project ( NSYSUKMU103 I-003 ) and the Grant of Biosignature in Colorectal Cancers, Academia Sinica , Taiwan. This study was also supported partially by Aim for the Top 500 Universities grant, Kaohsiung Medical University , Taiwan ( KMU-TP103C00 and KMU-DT103005 ). Publisher Copyright: {\textcopyright} 2015 Elsevier Ltd. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",
year = "2015",
month = jul,
day = "1",
doi = "10.1016/j.tvjl.2015.02.007",
language = "English",
volume = "205",
pages = "107--109",
journal = "Veterinary Journal",
issn = "1090-0233",
publisher = "Bailliere Tindall Ltd",
number = "1",
}
