TY - JOUR
T1 - Different sample preparation and detection methods for normal and lung cancer urinary proteome analysis.
AU - Sinchaikul, Supachok
AU - Tantipaiboonwong, Payungsak
AU - Sriyam, Supawadee
AU - Tzao, Ching
AU - Phutrakul, Suree
AU - Chen, Shui Tein
PY - 2010
Y1 - 2010
N2 - The urinary proteome is known to be a valuable field of study related to human physiological functions because many components in urine provide an alternative to blood plasma as a potential source of disease biomarkers useful in clinical diagnosis and therapeutic application. Due to the variability and complexity of urine, sample preparation is very important for decreasing the dynamic range of components and isolating specific urinary proteins prior to analysis. We discuss many useful sample preparation methods in this chapter, including those of lung cancer urine samples. In addition, protein detection methods are also crucial in visualizing protein profiles and for quantification of protein content in urine samples from both normal donor and lung cancer patients. This chapter also provides alternative choices of urine sample preparation and detection methods for selective use in urinary proteome analysis and for identifying urinary protein markers in lung cancer and other diseases.
AB - The urinary proteome is known to be a valuable field of study related to human physiological functions because many components in urine provide an alternative to blood plasma as a potential source of disease biomarkers useful in clinical diagnosis and therapeutic application. Due to the variability and complexity of urine, sample preparation is very important for decreasing the dynamic range of components and isolating specific urinary proteins prior to analysis. We discuss many useful sample preparation methods in this chapter, including those of lung cancer urine samples. In addition, protein detection methods are also crucial in visualizing protein profiles and for quantification of protein content in urine samples from both normal donor and lung cancer patients. This chapter also provides alternative choices of urine sample preparation and detection methods for selective use in urinary proteome analysis and for identifying urinary protein markers in lung cancer and other diseases.
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U2 - 10.1007/978-1-60761-711-2_5
DO - 10.1007/978-1-60761-711-2_5
M3 - Article
C2 - 20407942
AN - SCOPUS:77955479939
SN - 1064-3745
VL - 641
SP - 65
EP - 88
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -
