@article{9e5612f9b4eb413bae30b338de4a84bd,
title = "Dicer-mediated miR-200b expression contributes to cell migratory/invasive abilities and cancer stem cells properties of breast cancer cells",
abstract = "Distant metastasis is the leading cause of death in patients with breast cancer. Despite considerable treatment advances, the clinical outcomes of patients with metastatic breast cancer remain poor. CSCs can self-renew, enhancing cancer progression and metastasis. Dicer, a microRNA (miRNA) processing–related enzyme, is required for miRNA maturation. Imbalanced Dicer expression may be pivotal in cancer progression. However, whether and how Dicer affects the stemness of metastatic breast cancer cells remains unclear. Here, we hypothesized that Dicer regulates the migration, invasion, and stemness of breast cancer cells. We established highly invasive cell lines (MCF-7/I-3 and MDA-MB-231/I-3) and observed that Dicer expression was conspicuously lower in the highly invasive cells than in the parental cells. The silencing of Dicer significantly enhanced the cell migratory/invasive abilities and CSCs properties of the breast cancer cells. Conversely, the overexpression of Dicer in the highly invasive cells reduced their migration, invasion, and CSCs properties. Our bioinformatics analyses demonstrated that low Dicer levels were correlated with increased breast cancer risk. Suppression of Dicer inhibited miR-200b expression, whereas miR-200b suppression recovered Dicer knockdown–induced migration, invasion, and cancer stem cells (CSCs) properties of the breast cancer cells.",
keywords = "Cancer stem cells properties, Dicer, Invasion, Migration, Mir-200b",
author = "Hsu, {Tung Wei} and Chen, {Hsin An} and Liao, {Po Hsiang} and Su, {Yen Hao} and Chiu, {Ching Feng} and Huang, {Chih Yang} and Lin, {Yu Jung} and Hung, {Chih Chiang} and Yeh, {Ming Hsin} and Sung, {Shian Ying} and Su, {Chih Ming}",
note = "Funding Information: This study was supported by a study project grant from the Taipei Medical University-Shuang Ho Hospital, Ministry of Health and Welfare grant from Taiwan (103TMU-SHH-26 and 104TMU-SHH-01-2); Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan (DP2-110-21121-01-K-03; DP2-111-21121-01-C-08--01; DP2-111-21121-01-C-08-02; DP2-111-21121-01-C-08-03 and DP2-110-21121-01-K-03); Ministry of Health and Welfare (MOHW110-TDU-B-212-124007; MOHW110-B-212-144026 and MOHW111-TDU-B-221-114002); Taipei Medical University Hospital (108-TMUH-SP-01) and Ministry of Science and Technology (MOST110-2314-B-038-136 and MOST110-2314-B-038-137-MY3). Publisher Copyright: {\textcopyright} 2022 Hsu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited",
year = "2022",
doi = "10.18632/aging.204205",
language = "English",
volume = "14",
pages = "6520--6536",
journal = "Aging",
issn = "1945-4589",
publisher = "US Administration on Aging",
number = "16",
}