摘要
Treatment of cultured bovine carotid artery endothelial cells with 10-7M plasmin increased the cellular diacylglycerol which was determined by the formation of [3H]palmitate-labeled diacylglycerol and diacylglycerol mass. Upon the stimulation with plasmin, a gradual increase in diacylglycerol formation was observed within 20 min then slightly declined. The maximal effect during the 1-h time course study was 45 and 55% increases in [3H]palmitate-labeled diacylgycerol and diacylglycerol mass, respectively, at 20 min after plasmin treatment. Formation of phosphatidylethanol was also studied in [3H]palmitate-prelabeled cells in the presence of ethanol. Treatment with plasmin for 20 min induced a significant 45% increase in phosphatidylethanol formation. The present results indicate that the plasmin-induced diacylglycerol formation in endothelial cells was at least in part mediated through the phospholipase D activation.
原文 | 英語 |
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頁(從 - 到) | 89-95 |
頁數 | 7 |
期刊 | Thrombosis Research |
卷 | 76 |
發行號 | 1 |
DOIs | |
出版狀態 | 已發佈 - 10月 1 1994 |
對外發佈 | 是 |
ASJC Scopus subject areas
- 血液學