Diacylglycerol formation induced by human plasmin in cultured endothelial cells

Wen Chang Chang, Guey Yueh Shi, Ming T. Lin, Chauyin J. Jen, Lih Yuh C. Wing, Hua Lin Wu

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1 引文 斯高帕斯(Scopus)


Treatment of cultured bovine carotid artery endothelial cells with 10-7M plasmin increased the cellular diacylglycerol which was determined by the formation of [3H]palmitate-labeled diacylglycerol and diacylglycerol mass. Upon the stimulation with plasmin, a gradual increase in diacylglycerol formation was observed within 20 min then slightly declined. The maximal effect during the 1-h time course study was 45 and 55% increases in [3H]palmitate-labeled diacylgycerol and diacylglycerol mass, respectively, at 20 min after plasmin treatment. Formation of phosphatidylethanol was also studied in [3H]palmitate-prelabeled cells in the presence of ethanol. Treatment with plasmin for 20 min induced a significant 45% increase in phosphatidylethanol formation. The present results indicate that the plasmin-induced diacylglycerol formation in endothelial cells was at least in part mediated through the phospholipase D activation.

頁(從 - 到)89-95
期刊Thrombosis Research
出版狀態已發佈 - 10月 1 1994

ASJC Scopus subject areas

  • 血液學


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