TY - JOUR
T1 - Development of pullulan bioactive packaging and evaluation on frozen Tilapia fillets
AU - Huang, Yi Tsun
AU - Lin, Shin Ping
AU - Hsu, Kai Di
AU - Cheng, Kuan Chen
PY - 2017/4/1
Y1 - 2017/4/1
N2 - In this study, trisodium trimetaphosphate (STMP) was used as a crosslinking agent to improve the water resistance and physical properties of pullulan. The decreasing O-H stretching vibration(3, 300 cm'1) of crosslinking pullulan in FT-IR spectra indicate a higher degree of crosslinking was formed with increasing concentration of crosslinking reagent. In addition, nisin, a bacteriocin that produced by Lactococcus lactics, was incorporated into the pullulan film to develop an antibacterial food package system. The antibacterial activity of active pullulan film was also tested for common strains: E. coli, S. aureus, L. monocytogenes and V. parahaemolyticus, utilizing in vitro and on the meat. Results demonstrated that nisin and EDTA incorporated pullulan hydrogel could at least reduce 1.12 and 2.72 log of cfu in the S. aureus and L. innocua. In the frozen tilapia food package, frozen tilapia with active pullulan package could surely inhibit the growth of L. innocua and V. parahaemolyticus by at least 2.11 and 1.66 log of cfu in a 10-day test. In summary, the crosslinking strategy effectively improve the hydrophobic property of pullulan, and expect the active pullulan-nisin absorbend pad could apply to food package to extend the shelf life of meat.
AB - In this study, trisodium trimetaphosphate (STMP) was used as a crosslinking agent to improve the water resistance and physical properties of pullulan. The decreasing O-H stretching vibration(3, 300 cm'1) of crosslinking pullulan in FT-IR spectra indicate a higher degree of crosslinking was formed with increasing concentration of crosslinking reagent. In addition, nisin, a bacteriocin that produced by Lactococcus lactics, was incorporated into the pullulan film to develop an antibacterial food package system. The antibacterial activity of active pullulan film was also tested for common strains: E. coli, S. aureus, L. monocytogenes and V. parahaemolyticus, utilizing in vitro and on the meat. Results demonstrated that nisin and EDTA incorporated pullulan hydrogel could at least reduce 1.12 and 2.72 log of cfu in the S. aureus and L. innocua. In the frozen tilapia food package, frozen tilapia with active pullulan package could surely inhibit the growth of L. innocua and V. parahaemolyticus by at least 2.11 and 1.66 log of cfu in a 10-day test. In summary, the crosslinking strategy effectively improve the hydrophobic property of pullulan, and expect the active pullulan-nisin absorbend pad could apply to food package to extend the shelf life of meat.
KW - Active packaging
KW - Listeria innocua
KW - Nisin
KW - Pullulan
KW - Vibrio parahaemolyticus
UR - http://www.scopus.com/inward/record.url?scp=85046972634&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85046972634&partnerID=8YFLogxK
U2 - 10.6578/TJACFS.2017.012
DO - 10.6578/TJACFS.2017.012
M3 - Article
AN - SCOPUS:85046972634
SN - 1605-2471
VL - 55
SP - 112
EP - 119
JO - Taiwanese Journal of Agricultural Chemistry and Food Science
JF - Taiwanese Journal of Agricultural Chemistry and Food Science
IS - 2
ER -