DEVELOPMENT OF MICROWELL ARRAY FOR CELL TRAPPING AND SHRNA DELIVERY USING ELECTROPORATION

Han Yun Hsieh, Che Yi Lee, Jian Chie Chao, Yu Jui Fan, Horn Jiunn Sheen

研究成果: 書貢獻/報告類型會議貢獻

摘要

First, we use microelectromechanical system (MEMS) technology to fabricate devices with different geometric structures on indium tin oxide (ITO) glass. The devices will be connected to an external electrical signal source to make the cells captured into the etched holes with negative dielectrophoresis (nDEP) to achieve cell manipulation. Second, we modulate the frequency of the electrical signal to achieve cell electroporation, and stain the cells with CFDA-SE and calcein to verify that after electropration, substances can be delivered from inside or outside the cell, and to find the optimal device for shRNA delivery.
原文英語
主出版物標題MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
發行者Chemical and Biological Microsystems Society
頁面1231-1232
頁數2
ISBN(電子)9781733419048
出版狀態已發佈 - 2022
事件26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 - Hybrid, Hangzhou, 中國
持續時間: 10月 23 202210月 27 2022

出版系列

名字MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences

會議

會議26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022
國家/地區中國
城市Hybrid, Hangzhou
期間10/23/2210/27/22

ASJC Scopus subject areas

  • 化學工程(雜項)
  • 生物工程
  • 一般化學
  • 控制與系統工程

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