TY - JOUR
T1 - Development of Decellularized Cornea by Organic Acid Treatment for Corneal Regeneration
AU - Lin, Hung Jun
AU - Wang, Tsung Jen
AU - Li, Ting Wei
AU - Chang, Yao Yuan
AU - Sheu, Ming Thau
AU - Huang, Yi You
AU - Liu, Der Zen
N1 - Funding Information:
This work was supported by the Ministry of Science and Technology, Taiwan under Grant (MOST105-2221-E-038-003-MY3); and the Council of Agriculture, Taiwan under Grant (106AS-6.2.1-ST-a1).
Publisher Copyright:
© Copyright 2019, Mary Ann Liebert, Inc., publishers.
PY - 2019/4/1
Y1 - 2019/4/1
N2 - Because of the lack of donor corneas, an important area of research is the development of bioengineered corneal scaffolds to treat corneal blindness. Decellularized cornea has become a prominent area of research to satisfy the clinical demand. However, the limitation of its application is that a suitable decellularization procedure has not been developed. Organic acids are naturally occurring constituents in animal tissues and plants, and could be safely neutralized into harmless salts. In this study, we developed decellularized porcine corneal (dPC) scaffolds that were prepared by organic acid treatment. Cell removal and intact extracellular matrix preservation were evidenced by histological and biochemical quantitative analysis, and the dPC scaffolds showed porous parallel lamellar microstructure and excellent biomechanical properties. In vitro cell culture demonstrated that the dPC scaffolds had good biocompatibility, and the porous microstructure provided an ideal space for the growth of stroma keratocytes. Moreover, in vivo implantation revealed ideal reepithelialization, stromal recellularization, and complete transparency during the full follow-up period. Thus, dPC scaffolds that were prepared by organic acid treatment could be a promising biological material for use in corneal transplantation.
AB - Because of the lack of donor corneas, an important area of research is the development of bioengineered corneal scaffolds to treat corneal blindness. Decellularized cornea has become a prominent area of research to satisfy the clinical demand. However, the limitation of its application is that a suitable decellularization procedure has not been developed. Organic acids are naturally occurring constituents in animal tissues and plants, and could be safely neutralized into harmless salts. In this study, we developed decellularized porcine corneal (dPC) scaffolds that were prepared by organic acid treatment. Cell removal and intact extracellular matrix preservation were evidenced by histological and biochemical quantitative analysis, and the dPC scaffolds showed porous parallel lamellar microstructure and excellent biomechanical properties. In vitro cell culture demonstrated that the dPC scaffolds had good biocompatibility, and the porous microstructure provided an ideal space for the growth of stroma keratocytes. Moreover, in vivo implantation revealed ideal reepithelialization, stromal recellularization, and complete transparency during the full follow-up period. Thus, dPC scaffolds that were prepared by organic acid treatment could be a promising biological material for use in corneal transplantation.
KW - cornea
KW - corneal transplantation
KW - decellularized scaffold
KW - extracellular matrix
KW - xenograft
KW - Corneal Diseases/surgery
KW - Tissue Engineering/methods
KW - Extracellular Matrix
KW - Corneal Transplantation/methods
KW - Animals
KW - Swine
KW - Tissue Scaffolds/chemistry
KW - Cornea/cytology
KW - Epithelium, Corneal/cytology
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UR - http://www.scopus.com/inward/citedby.url?scp=85064699130&partnerID=8YFLogxK
U2 - 10.1089/ten.tea.2018.0162
DO - 10.1089/ten.tea.2018.0162
M3 - Article
C2 - 30244654
AN - SCOPUS:85064699130
SN - 1937-3341
VL - 25
SP - 652
EP - 662
JO - Tissue Engineering - Part A
JF - Tissue Engineering - Part A
IS - 7-8
ER -