@article{0def32d693b04d2c9649b1318bbb7458,
title = "Development of an enrichment-free one-pot sample preparation and ultra-high performance liquid chromatography-tandem mass spectrometry method to identify Immunoglobulin A1 hinge region O-glycoforms for Immunoglobulin A nephropathy",
abstract = "Immunoglobulin A nephropathy (IgAN) is a highly prevalent autoimmune renal disease. Human IgA1 with galactose deficiency in the hinge region (HR) has been identified as an autoantigen for this disease. Therefore, analyzing IgA1 HR glycoforms in biofluids is important for biomarker discovery. Herein, an analytical method that includes one-pot sample preparation with unbiased plasma IgA purification, dual internal standard addition, and sensitive ultra-high-performance liquid chromatography-triple quadrupole tandem mass spectroscopy (UHPLC-QqQ-MS/MS) was developed. Targeted O-glycopeptides detection was performed in pooled plasma with the validation of theoretical retention times, enzymatic treatment outcomes, product ion scans, and signal repeatability. A total of 42 IgA1 O-glycopeptides with N-acetylgalactosamines, galactoses, and sialic acids were determined from 8 µL of plasma. The newly developed method was applied to plasma samples from 16 non-IgAN controls and 19 IgAN patients. Comparing the 42 targets, 16 IgA1 HR O-glycopeptides were statistically different between the two groups (p<0.05). Decreased sialylation was identified in the IgA1 hinge region of IgAN patients, which was also correlated with the estimated glomerular filtration rate (eGFR). The developed method is sensitive and precise and can be used to identify plasma biomarkers for IgA nephropathy.",
keywords = "Biomarkers, IgA nephropathy, Immunoglobulin A, Liquid chromatography-mass spectrometry, O-glycosylation",
author = "Chen, {Hsiao Fan} and Kao, {Chih Chin} and Ka, {Shuk Man} and Wang, {San Yuan} and Chen, {Michael X.} and Chen, {Guan Yuan} and Weng, {Te I.} and Lai, {Ruo Yun} and Yeh, {Shu Ching} and Lin, {Yen Chung} and Chen, {Hsi Hsien} and Chang, {Wei Chiao} and Wu, {Mai Szu} and Tsai, {I. Lin}",
note = "Funding Information: This study was funded by the Ministry of Science and Technology, Taiwan (grant numbers 107-2314-B-038-019-MY3 and 108-2320-B-038-060-MY3 ). The authors thank the technical support provided by the TMU Core Facility and would like to acknowledge the academic and science graphic illustration service provided by the TMU Office of Research and Development. We also thank Dr. Ann Chen for providing instructions on clinical applications. Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",
year = "2022",
month = dec,
day = "6",
doi = "10.1016/j.chroma.2022.463589",
language = "English",
volume = "1685",
journal = "Journal of Chromatography A",
issn = "0021-9673",
}
