Development of a water refractive index-matched microneedle integrated into a light sheet microscopy system for continuous embryonic cell imaging

Yu Jui Fan, Han Yun Hsieh, Yen Ru Huang, Chieh Tsao, Chia Ming Lee, Hidetoshi Tahara, Yi Chun Wu, Horn Jiunn Sheen, Bi Chang Chen

研究成果: 雜誌貢獻文章同行評審

2 引文 斯高帕斯(Scopus)

摘要

In this study, microneedle-integrated light sheet microscopy (LSM) was developed for trapping and continuously imaging embryos of Caenorhabditis elegans with subcellular resolution. To reduce aberrations when the light sheet was propagated into the device, a microneedle was fabricated using a transparent, water refractive index-matched polymer. It was proven that when the light sheet emerged from the water-immersed objective and penetrated through the microneedle with a circular surface, even with a non-perpendicular incident angle, fewer aberrations were found. An embryo was injected into and trapped at the tip of the microneedle, which was positioned at the interrogation window of the LSM apparatus with the image plane perpendicular to the light sheet, and this setup was used to sequentially acquire embryo images. By applying the light sheet, higher-resolution, higher-contrast images were obtained. The system also showed low photobleaching and low phototoxicity to embryos of C. elegans. Furthermore, three-dimensional embryo images with a whole field of view of the microneedle could be achieved by stitching together images and reconstructing sequential two-dimensional embryo images.

原文英語
頁(從 - 到)584-591
頁數8
期刊Lab on a Chip
22
發行號3
DOIs
出版狀態已發佈 - 2月 7 2022

ASJC Scopus subject areas

  • 生物工程
  • 生物化學
  • 一般化學
  • 生物醫學工程

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