TY - JOUR
T1 - Development of a simple fluorescence sensing system for carbonyl stress in urine
AU - Huang, Wei Jan
AU - Sun, Yin Tung
AU - Lee, Wei Han
AU - Lin, Po Yeh
AU - Chen, Chien Ming
N1 - Funding Information:
This work was financially supported by the National Taipei University of Technology-Taipei Medical University Joint Research Program (NTUT‐TMU‐103‐12), and the Ministry of Science and Technology of Taiwan (109-2637-E-027-002-). The authors would like to thank Prof. Jen-Ai Lee at Taipei Medical University for her invaluable assistance.
Publisher Copyright:
© 2021 M Y U Scientific Publishing Division. All rights reserved.
PY - 2021
Y1 - 2021
N2 - In this study, we developed a simple fluorescence sensing system to rapidly measure the total carbonyl stress in urine because carbonyls may form advanced glycation end products, leading to diseases. The traditional method for detecting carbonyl stress has been high-performance liquid chromatography (HPLC), which detects glyoxal, methylglyoxal, and 3-deoxyglucosone contents. The excitation and emission wavelengths used in the sensing system to monitor aldehyde content in urine were 340 and 500 nm, respectively. Rat urine samples were derivatized with 5,6-diamino-2,4-hydroxypyrimidine sulfate dihydrate. A 20 µL sample was added to a carrier composed of polydimethylsiloxane for testing, and only 1 s was required for detection. According to the fluorescence intensity results, the calibration curve for the total content of all aldehydes in the urine had a good degree of linearity with the fluorescence intensity (R2 = 0.9879). In addition, the urine of diabetic and healthy rats was tested, and the correlation coefficient between the total carbonyl content detected by the simple fluorescence system and HPLC reached 0.8132. Collectively, our findings demonstrated that our system can rapidly measure total carbonyl stress in urine with a small sample volume.
AB - In this study, we developed a simple fluorescence sensing system to rapidly measure the total carbonyl stress in urine because carbonyls may form advanced glycation end products, leading to diseases. The traditional method for detecting carbonyl stress has been high-performance liquid chromatography (HPLC), which detects glyoxal, methylglyoxal, and 3-deoxyglucosone contents. The excitation and emission wavelengths used in the sensing system to monitor aldehyde content in urine were 340 and 500 nm, respectively. Rat urine samples were derivatized with 5,6-diamino-2,4-hydroxypyrimidine sulfate dihydrate. A 20 µL sample was added to a carrier composed of polydimethylsiloxane for testing, and only 1 s was required for detection. According to the fluorescence intensity results, the calibration curve for the total content of all aldehydes in the urine had a good degree of linearity with the fluorescence intensity (R2 = 0.9879). In addition, the urine of diabetic and healthy rats was tested, and the correlation coefficient between the total carbonyl content detected by the simple fluorescence system and HPLC reached 0.8132. Collectively, our findings demonstrated that our system can rapidly measure total carbonyl stress in urine with a small sample volume.
KW - Carbonyl stress
KW - Fluorescence
KW - Methylglyoxal
KW - Urine
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U2 - 10.18494/SAM.2021.3533
DO - 10.18494/SAM.2021.3533
M3 - Article
AN - SCOPUS:85116792917
SN - 0914-4935
VL - 33
SP - 3489
EP - 3497
JO - Sensors and Materials
JF - Sensors and Materials
IS - 10
ER -
