摘要
The stem with hook of Uncaria rhynchophylla (Chinese herbal name Gou-Teng) is a traditional Chinese medicine that has been ethnopharmacologically used to extinguish wind and clean interior heat. Rhynchophylline (RHY), a tetracyclic oxindole alkaloid isolated from U. rhynchophylla, displays significant antineuroinflammatory effects. However, there is no evidence to indicate that rhynchophylline can cross the blood-brain barrier and be detected in the brain. In this study, an in vivo microdialysis sampling method coupled with UPLC/MS/MS was employed for the continuous simultaneous monitoring of unbound RHY in rat blood and brain. The precursor ion→product ion transition at m/z 385.2→160.0 for rhynchophylline was monitored. A calibration curve gave good linearity (r>0.996) over the concentration range from 0.5 to 1000ng/mL. The results demonstrated that rhynchophylline could be detected in the brain and plasma from 15min to 6h after its administration (1 or 10mg/kg, i.v.). All the pharmacokinetic parameters of rhynchophylline in the brain and plasma were obtained. These results show that rhynchophylline can cross the blood-brain barrier and they provide useful clinical information.
原文 | 英語 |
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頁(從 - 到) | 901-906 |
頁數 | 6 |
期刊 | Biomedical Chromatography |
卷 | 28 |
發行號 | 6 |
DOIs | |
出版狀態 | 已發佈 - 6月 2014 |
對外發佈 | 是 |
ASJC Scopus subject areas
- 藥物發現
- 分析化學
- 分子生物學
- 生物化學
- 臨床生物化學
- 藥理