摘要
A simple and sensitive high-performance liquid chromatographic method involving fluorescence detection was developed for the determination of mephenoxalone in human plasma. A Cosmosil 5C18-MS column (250 mm×4.6 mm I.D., 5 μm) was used as stationary phase and the mobile phase consisted of water-acetic acid-acetonitrile (200:1:300) at a flow-rate of 1.0 ml/min. The fluorescence absorbance was monitored at 280 nm for excitation wavelength and 310 nm for emission wavelength. Temperature control was kept at 40°C for the column. The limit of quantitation achieved was 10 ng/ml, and the standard curve was found to be linear in the concentration range of 10-10 000 ng/ml. Under these analytical conditions, a sufficient mephenoxalone plasma concentration profile could be obtained for pharmacokinetic study.
原文 | 英語 |
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頁(從 - 到) | 91-97 |
頁數 | 7 |
期刊 | Journal of Chromatography B: Biomedical Sciences and Applications |
卷 | 759 |
發行號 | 1 |
DOIs | |
出版狀態 | 已發佈 - 8月 5 2001 |
ASJC Scopus subject areas
- 一般化學