Delayed reproductive death in x-irradiated chinese hamster ovary cells

W. P. Chang, J. B. Little

研究成果: 雜誌貢獻文章同行評審

167 引文 斯高帕斯(Scopus)

摘要

The cytotoxic effects of X-irradiation may be delayed for many generations of replication in mammalian cells. In addition to a reduced cloning efficiency, progeny of surviving Chinese hamster ovary (CHO) cells isolated after 12-34 population doublings postirradiation showed a variety of abnormalities including lower attachment ability to culture dishes and slower cell cycle progression. When these progeny were seeded as single cells 12-23 generations after irradiation, they formed a higher fraction of abortive colonies and of non-homogeneous colonies containing giant cells. All of these characteristics were evident whether progeny cells were subcultured by trypsinization or by mitotic selection. These results suggest that residual damage may be carried by surviving progeny of irradiated cells over many mitotic cycles; this damage is eventually expressed in terms of pleomorphic changes leading to delayed reproductive failure.

原文英語
頁(從 - 到)483-496
頁數14
期刊International Journal of Radiation Biology
60
發行號3
DOIs
出版狀態已發佈 - 1991
對外發佈

ASJC Scopus subject areas

  • 放射與超音波技術
  • 放射學、核子醫學和影像學

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