TY - JOUR
T1 - Decreased suppression of interleukin-6 after treatment with medroxyprogesterone acetate and danazol in endometrial stromal cells of women with adenomyosis
AU - Yang, Jehn Hsiahn
AU - Chen, Mei Jou
AU - Wu, Ming Yih
AU - Chen, Yi Chun
AU - Yang, Yu Shih
AU - Ho, Hong Nerng
N1 - Funding Information:
Supported by grant NSC93-2314-B002-159 from the National Science Council of the Republic of China and the research grant NTUH94-S72 from National Taiwan University Hospital.
PY - 2006/11/1
Y1 - 2006/11/1
N2 - Objective: To examine changes in local cytokine production in the eutopic endometrium of adenomyosis after treatment with medroxyprogesterone acetate (MPA) and danazol. Design: Prospective cohort study. Setting: Tertiary university hospital. Patient(s): Twenty-four women with adenomyosis (study group) and 22 women without adenomyosis (control group). Intervention(s): Eutopic endometrium was separated into single endometrial stromal cells (ESCs), and was cultured in vitro alone, with MPA (10-7 M), or with danazol (10-7 M) for 8 days. Main Outcome Measure(s): Messenger RNA expression of interleukin (IL)-6, IL-8, and tumor necrosis factor-α in ESCs, and their concentrations in the supernatants of ESCs. Result(s): A significant decrease of IL-6 concentration in the supernatant, measured by ELISA, was found with time of ESCs cultured with MPA and danazol in the control group, but not in adenomyosis. The IL-6 mRNA in ESCs determined by real-time quantitative polymerase chain, as well as its concentration in the supernatant, was much higher in adenomyosis than that in the control group after treatment with MPA and danazol for 8 days. Conclusion(s): Medroxyprogesterone acetate and danazol appeared to have a decreased effect on the suppression of IL-6 liberated by ESCs in adenomyosis. This aberrant secretion of IL-6 might play a role in the formation of ectopic endometrial implants in adenomyosis.
AB - Objective: To examine changes in local cytokine production in the eutopic endometrium of adenomyosis after treatment with medroxyprogesterone acetate (MPA) and danazol. Design: Prospective cohort study. Setting: Tertiary university hospital. Patient(s): Twenty-four women with adenomyosis (study group) and 22 women without adenomyosis (control group). Intervention(s): Eutopic endometrium was separated into single endometrial stromal cells (ESCs), and was cultured in vitro alone, with MPA (10-7 M), or with danazol (10-7 M) for 8 days. Main Outcome Measure(s): Messenger RNA expression of interleukin (IL)-6, IL-8, and tumor necrosis factor-α in ESCs, and their concentrations in the supernatants of ESCs. Result(s): A significant decrease of IL-6 concentration in the supernatant, measured by ELISA, was found with time of ESCs cultured with MPA and danazol in the control group, but not in adenomyosis. The IL-6 mRNA in ESCs determined by real-time quantitative polymerase chain, as well as its concentration in the supernatant, was much higher in adenomyosis than that in the control group after treatment with MPA and danazol for 8 days. Conclusion(s): Medroxyprogesterone acetate and danazol appeared to have a decreased effect on the suppression of IL-6 liberated by ESCs in adenomyosis. This aberrant secretion of IL-6 might play a role in the formation of ectopic endometrial implants in adenomyosis.
KW - Adenomyosis
KW - danazol
KW - endometrial stromal cell
KW - interleukin-6
KW - medroxyprogesterone acetate
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U2 - 10.1016/j.fertnstert.2006.04.034
DO - 10.1016/j.fertnstert.2006.04.034
M3 - Article
C2 - 16989825
AN - SCOPUS:33750193673
SN - 0015-0282
VL - 86
SP - 1459
EP - 1465
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 5
ER -