De novo sequencing of circulating miRNAs identifies novel markers predicting clinical outcome of locally advanced breast cancer

Xiwei Wu, George Somlo, Yang Yu, Melanie R. Palomares, Arthur X. Li, Weiying Zhou, Amy Chow, Yun Yen, John J. Rossi, Harry Gao, Jinhui Wang, Yate Ching Yuan, Paul Frankel, Sierra Li, Kimlin T. Ashing-Giwa, Guihua Sun, Yafan Wang, Robin Smith, Kim Robinson, Xiubao RenShizhen E. Wang

研究成果: 雜誌貢獻文章同行評審

191 引文 斯高帕斯(Scopus)


Background: MicroRNAs (miRNAs) have been recently detected in the circulation of cancer patients, where they are associated with clinical parameters. Discovery profiling of circulating small RNAs has not been reported in breast cancer (BC), and was carried out in this study to identify blood-based small RNA markers of BC clinical outcome.Methods: The pre-treatment sera of 42 stage II-III locally advanced and inflammatory BC patients who received neoadjuvant chemotherapy (NCT) followed by surgical tumor resection were analyzed for marker identification by deep sequencing all circulating small RNAs. An independent validation cohort of 26 stage II-III BC patients was used to assess the power of identified miRNA markers.Results: More than 800 miRNA species were detected in the circulation, and observed patterns showed association with histopathological profiles of BC. Groups of circulating miRNAs differentially associated with ER/PR/HER2 status and inflammatory BC were identified. The relative levels of selected miRNAs measured by PCR showed consistency with their abundance determined by deep sequencing. Two circulating miRNAs, miR-375 and miR-122, exhibited strong correlations with clinical outcomes, including NCT response and relapse with metastatic disease. In the validation cohort, higher levels of circulating miR-122 specifically predicted metastatic recurrence in stage II-III BC patients.Conclusions: Our study indicates that certain miRNAs can serve as potential blood-based biomarkers for NCT response, and that miR-122 prevalence in the circulation predicts BC metastasis in early-stage patients. These results may allow optimized chemotherapy treatments and preventive anti-metastasis interventions in future clinical applications.

期刊Journal of Translational Medicine
出版狀態已發佈 - 3月 8 2012

ASJC Scopus subject areas

  • 生物化學、遺傳與分子生物學 (全部)


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