Cyclic diguanylic acid behaves as a host molecule for planar intercalators

Yen Chywan Liaw, Yi Gui Gao, Howard Robinson, George M. Sheldrick, L. A.J.M. Sliedregt, Gijs A. van der Marel, Jacques H. van Boom, Andrew H.J. Wang

研究成果: 雜誌貢獻文章同行評審

36 引文 斯高帕斯(Scopus)


Cyclic ribodiguanylic acid, c-(GpGp), is the endogenous effector regulator of cellulose synthase. Its three-dimensional structure from two different crystal forms (tetragonal and trigonal) has been determined by X-ray diffraction analysis at 1 Å resolution. In both crystal forms, two independent c-(GpGp) molecules associate with each other to form a self-intercalated dimer. A hydrated cobalt ion is found to coordinate to two N7 atoms of adjacent guanines, forcing these two guanines to destack with a large dihedral angle (32°), in the dimer of the tetragonal form. This metal coordination mechanism may be relevant to that of the anticancer drug cisplatin. Moreover, c-(GpGp) exhibits unusual spectral properties not seen in any other cyclic dinucleotide. It interacts with planar organic intercalator molecules in ways similar to double helical DNA. We propose a cage-like model consisting of a tetrameric c-(GpGp) aggregate in which a large cavity ('host') is generated to afford a binding site for certain planar intercalators ('guests').

頁(從 - 到)223-227
期刊FEBS Letters
出版狀態已發佈 - 5月 21 1990

ASJC Scopus subject areas

  • 生物物理學
  • 結構生物學
  • 生物化學
  • 分子生物學
  • 遺傳學
  • 細胞生物學


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