Cuphiin D1, the macrocyclic hydrolyzable tannin induced apoptosis in HL-60 cell line

Ching Chiung Wang, Lih Geeng Chen, Ling Ling Yang

研究成果: 雜誌貢獻文章同行評審

34 引文 斯高帕斯(Scopus)


Cuphiin D1 (CD1), a new macrocyclic hydrolyzable tannin isolated from Cuphea hyssopifolia, has been shown to exert antitumor activity both in vitro and in vivo. In this study, we explored the mechanism of the CD1-induced antitumor effect on human promyelocytic leukemia (HL-60) cells. The results showed that CD1 induced cytotoxicity in HL-60 cells and the IC50 was 16 μM after 36 h treatment. HL-60 cells treated with CD1 for 36 h decreased the uptake of [3H]-labeled thymidine, uridine and leucine in a dose dependent manner. Electron micrographs demonstrated that HL-60 cells treated with 16 μM CD1 for 36 h exhibited chromatin condensation, indicating the apoptosis occurrence. Flow cytometric analysis demonstrated the presence of apoptotic cells with low DNA content, a decrease of cell population at G2/M phase, and a concomitant increase of cell population at G1 phase. CD1 also caused DNA fragmentation and inhibited Bcl-2 expression in the HL-60 cells. These results suggest that the inhibition of Bcl-2 expression in HL-60 cell might account for the mechanism of CD1-induced apoptosis. (C) 2000 Elsevier Science Ireland Ltd.
頁(從 - 到)77-83
期刊Cancer Letters
出版狀態已發佈 - 2月 28 2000

ASJC Scopus subject areas

  • 癌症研究
  • 分子生物學
  • 腫瘤科


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