CSE1L modulates ras-induced cancer cell invasion: Correlation of K-ras mutation and CSE1L expression in colorectal cancer progression

Ming Chung Jiang, Chung Min Yeh, Cheng Jeng Tai, Hung Chang Chen, Shu Hui Lin, Tzu Cheng Su, Shing Chuan Shen, Woan Ruoh Lee, Ching Fong Liao, Li Tzu Li, Ching Hsiao Lee, Ying Chun Chen, Kun Tu Yeh, Chun Chao Chang

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17 引文 斯高帕斯(Scopus)

摘要

Background: Ras plays an important role in colorectal cancer progression. CSE1L (chromosome segregation 1-like) gene maps to 20q13, a chromosomal region that correlates with colorectal cancer development. We investigated the association of CSE1L with Ras in colorectal cancer progression. Methods: The effect of CSE1L on metastasis-stimulating activity of Ras was studied in an animal model with tumor cells expressing CSE1L-specific shRNA and v-H-Ras. CSE1L expression was evaluated by the immunohistochemical analysis of 127 surgically resected colorectal tumors. K-Ras mutations were analyzed by direct sequencing. Results: CSE1L knockdown reduced Ras-induced metastasis of B16F10 melanoma cells in C57BL/6 mice. v-H-Ras expression altered the cellular trafficking of CSE1L and increased CSE1L secretion. Most colorectal tumors were positive for CSE1L staining (98.4%, 125 of 127). Colorectal tumors with K-Ras mutation or high cytoplasmic CSE1L expression were correlated with T status (depth of tumor penetration; P =.004), stage (P =.004), and lymph node metastasis (P =.019). Conclusions: CSE1L may be a target for treating Ras-associated tumors. Analysis of K-Ras mutation and CSE1L expression may provide valuable clinical and pathological information to aid in the determination of treatment options for colorectal cancer.

原文英語
頁(從 - 到)418-427
頁數10
期刊American Journal of Surgery
206
發行號3
DOIs
出版狀態已發佈 - 9月 2013

ASJC Scopus subject areas

  • 手術

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