TY - JOUR
T1 - Crystallization of peptidase T from Salmonella typhimurium
AU - Håkansson, Kjell
AU - Broder, Dan
AU - Wang, Andrew H.J.
AU - Miller, Charles G.
PY - 2000
Y1 - 2000
N2 - Aminotripeptidase (peptidase T) from Salmonella typhimurium and a derivative carrying a C-terminal His tag have been crystallized. In both cases, the space group was found to be C2, with a single molecule in the asymmetric unit. Crystals of the native peptidase T diffract to 2.9 Å, but a selenomethionine derivative of this protein did not yield good crystals. Crystals of the His-tag peptidase T diffracted to 2.6 Å, however, and could be used for the production of good-quality selenomethionine crystals. All 15 methionines, a native metal ion and two mercury reactive sites could be located and crystals suitable for MAD data collection have been produced.
AB - Aminotripeptidase (peptidase T) from Salmonella typhimurium and a derivative carrying a C-terminal His tag have been crystallized. In both cases, the space group was found to be C2, with a single molecule in the asymmetric unit. Crystals of the native peptidase T diffract to 2.9 Å, but a selenomethionine derivative of this protein did not yield good crystals. Crystals of the His-tag peptidase T diffracted to 2.6 Å, however, and could be used for the production of good-quality selenomethionine crystals. All 15 methionines, a native metal ion and two mercury reactive sites could be located and crystals suitable for MAD data collection have been produced.
UR - http://www.scopus.com/inward/record.url?scp=12944255857&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=12944255857&partnerID=8YFLogxK
U2 - 10.1107/S0907444900006375
DO - 10.1107/S0907444900006375
M3 - Article
C2 - 10930847
AN - SCOPUS:12944255857
SN - 0907-4449
VL - 56
SP - 924
EP - 926
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 7
ER -