@article{d6c8d1ba200a4232992095544e4ec79f,
title = "Crystal Structure of the C-Terminal Domain of a Flagellar Hook-Capping Protein from Xanthomonas campestris",
abstract = "The crystal structure of the C-terminal domain of a hook-capping protein FlgD from the plant pathogen Xanthomonas campestris (Xc) has been determined to a resolution of ca 2.5 {\AA} using X-ray crystallography. The monomer of whole FlgD comprises 221 amino acids with a molecular mass of 22.7 kDa, but the flexible N-terminus is cleaved for up to 75 residues during crystallization. The final structure of the C-terminal domain reveals a novel hybrid comprising a tudor-like domain interdigitated with a fibronectin type III domain. The C-terminal domain of XcFlgD forms three types of dimers in the crystal. In agreement with this, analytical ultracentrifugation and gel filtration experiments reveal that they form a stable dimer in solution. From these results, we propose that the Xc flagellar hook cap protein FlgD comprises two individual domains, a flexible N-terminal domain that cannot be detected in the current study and a stable C-terminal domain that forms a stable dimer.",
keywords = "FlgD, hook capping, Ig-like domain, Tudor-like domain, Xanthomonas campestris",
author = "Kuo, {Wei Ting} and Chin, {Ko Hsin} and Lo, {Wen Ting} and Wang, {Andrew H.J.} and Chou, {Shan Ho}",
note = "Funding Information: This work is supported by the Academic Excellence Pursuit grant from the Ministry of Education and by the National Science Council, Taiwan, ROC (grants 94-2113-M005-003 and 95-2113-M005-018) to S.-H. Chou. We thank the Core Facilities for Protein X-ray Crystallography in the Academia Sinica, Taiwan for help in crystal screening, the National Synchrotron Radiation Research Center (NSRRC) in Taiwan, and the SPring-8 Synchrotron facility in Japan for assistance of X-ray data collection. The NSRRC is a user facility supported by the National Science Council, Taiwan, ROC, and the Protein Crystallography Facility is supported by the National Research Program for Genomic Medicine, Taiwan, ROC.",
year = "2008",
month = aug,
day = "1",
doi = "10.1016/j.jmb.2008.05.083",
language = "English",
volume = "381",
pages = "189--199",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press Inc.",
number = "1",
}