Controlled Decomposable Hydrogel Triggered with a Specific Enzyme

You Ren Ji, Ya Hsiang Hsu, Ming Hua Syue, Ying Chu Wang, Shyr Yi Lin, Tsung Wei Huang, Tai Horng Young

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In this study, superabsorbent polyelectrolyte hydrogels were synthesized by cross-linking a nondegradable poly (allylamine hydrochloride) (PAH) and a recombinant protein with a specific enzymatic cleavage site. The recombinant protein was produced by E. coli with the pET-32b(+) plasmid, which is featured with the thioredoxin (Trx) gene containing a thrombin recognition site and a T7/lac hybrid promoter for high expression of recombinant protein. The swelling test shows that the composite hydrogel still maintained a high swelling ratio to 900% when 15% recombinant protein was cross-linked with PAH. The degradation test shows that such a PAH composite hydrogel could be decomposed by the addition of specific enzyme thrombin, which might lead to new biomedical applications of hydrogels needed to be decomposable by specific time not determined by the time period.
頁(從 - 到)3254-3261
期刊ACS Omega
出版狀態已發佈 - 2月 1 2022

ASJC Scopus subject areas

  • 化學 (全部)
  • 化學工程 (全部)


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