Conformations of the alternating (C-T)n sequence under neutral and low pH

T. N. Jaishree, Andrew H.J. Wang

研究成果: 雜誌貢獻文章同行評審

6 引文 斯高帕斯(Scopus)


The structures of the (C-T)n sequence at two different pHs have been analyzed by 500 MHz 2D-NMR using a modified DNA decamer d(CT[m5C]TCU[m5C]UCT) as a model system. The chemical modifications serve to perturb the monotonous C-T repeat, and consequently to yield a better chemical shift dispersion. The results reinforce our earlier suggestion that there are three major pH-depedent confonnational species: two antiparallel-stranded (APS) duplexes at pH 7 and pH 3, and a different structure near pH 5. Structural refinement of the decamer duplexes at pH = 7.5 and pH = 2.9 using 2D-NOE data suggests that the C : T or C+ : T base pairs are continuously stacked. Exchangeable proton NMR spectra at pH 7.5 and pH 2.9 are consistent with C : T or C+ : T base pairing schemes in which a water molecule bridges the two bases.

頁(從 - 到)139-144
期刊FEBS Letters
出版狀態已發佈 - 1月 10 1994

ASJC Scopus subject areas

  • 生物物理學
  • 結構生物學
  • 生物化學
  • 分子生物學
  • 遺傳學
  • 細胞生物學


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