Comparison of protein phosphatase inhibition activities and mouse toxicities of microcystins

Yi Min Chen, Zong-Huei Li, Shyh Jye Lee, Hsien Bin Huang, Rang Huang, Hong Nong Chou

研究成果: 雜誌貢獻文章同行評審

57 引文 斯高帕斯(Scopus)


Eight naturally purified microcystins (MCs), including MC-LR, -FR, -WR, -RR, [d-Asp3]MC-FR, -WR, -RR, and [Dha7]MC-RR were utilized to determine the effects of amino acid substitutions and modifications on MC-induced protein phosphatase inhibition activity and mouse toxicity. Catalytic subunits of protein phosphatase 1 (PP-1) and 2A (PP-2A) were purified and subjected to the inhibition assays, and intraperitoneal injection was used to administer MCs into mice for the toxicity assay. It is found that the replacement of the non-polar amino acid l-leucine at the second position of these heptacyclic peptide toxins by a polar l-arginine reduces their mouse toxicities and inhibitory activities against PP-1 and PP-2A to different extends. Demethylation of methyldehydroalanine (Mdha) at the seventh amino acid of MC-RR exhibits the least mouse toxicity and phosphatase inhibition. The loss of a methyl group on the common methylaspartic acid (MeAsp) at the third position of MC-FR, -WR, and -RR does not alter their toxicity levels, but dominantly reduces their activities in PP-1 inhibition compared to other substitutions or modifications. This suggests that the methyl group on MeAsp is also important for MCs inhibition. However, such a tendency is not observed for PP-2A. By comparing the LD50 values of the mouse toxicity assay and IC50 values of the PP-1 and PP-2A inhibition assay of eight MCs using linear regression, it is evident that the MC-induced toxicity is much more related to the inhibition of PP-2A than PP-1, which suggests that PP-2A inhibition may play a major role in the MC-induced mouse toxicity.
頁(從 - 到)742-746
出版狀態已發佈 - 6月 1 2006

ASJC Scopus subject areas

  • 毒理學


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