Comparative effect of lipoxygenase products of arachidonic acid on rat aortic smooth muscle cell migration

Junko Nakao; Toshiro Ooyama; Hideki Ito; Wen Chang Chang; Sei itsu Murota

doi:10.1016/0021-9150(82)90008-9

Comparative effect of lipoxygenase products of arachidonic acid on rat aortic smooth muscle cell migration

Junko Nakao, Toshiro Ooyama, Hideki Ito, Wen Chang Chang, Sei itsu Murota

研究成果: 雜誌貢獻 › 文章 › 同行評審

111 引文斯高帕斯（Scopus）

摘要

We investigated the effects of mono-hydroxyeicosatetraenoic acids (HETEs) and N-formyl-methionyl-leucyl-phenylalanine (F-Met-Leu-Phe) on rat aortic smooth muscle cell migration in modified Boyden chambers. 12-HETE showed the most potent stimulatory effect on smooth muscle cell migration among the mono-HETEs tested. The optimal concentrations for cell migration were 3 × 10^-15 and 3 × 10^-13 g/ml for 12-HETE and 10^-8 g/ml for 15-HETE. 5-HETE and F-Met-Leu-Phe were inactive with these cells. As 12-HETE is biosynthesized from arachidonic acid by the 12-lipoxygenase pathway in platelets and macrophages, and 15-HETE by the 15-lipoxygenase pathway in granulocytes, the present results indicate an important role for such cells in the early phase of atherosclerosis.

原文	英語
頁（從 - 到）	339-342
頁數	4
期刊	Atherosclerosis
卷	44
發行號	3
DOIs	https://doi.org/10.1016/0021-9150(82)90008-9
出版狀態	已發佈 - 9月 1982
對外發佈	是

ASJC Scopus subject areas

心臟病學與心血管醫學

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10.1016/0021-9150(82)90008-9

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title = "Comparative effect of lipoxygenase products of arachidonic acid on rat aortic smooth muscle cell migration",

abstract = "We investigated the effects of mono-hydroxyeicosatetraenoic acids (HETEs) and N-formyl-methionyl-leucyl-phenylalanine (F-Met-Leu-Phe) on rat aortic smooth muscle cell migration in modified Boyden chambers. 12-HETE showed the most potent stimulatory effect on smooth muscle cell migration among the mono-HETEs tested. The optimal concentrations for cell migration were 3 × 10-15 and 3 × 10-13 g/ml for 12-HETE and 10-8 g/ml for 15-HETE. 5-HETE and F-Met-Leu-Phe were inactive with these cells. As 12-HETE is biosynthesized from arachidonic acid by the 12-lipoxygenase pathway in platelets and macrophages, and 15-HETE by the 15-lipoxygenase pathway in granulocytes, the present results indicate an important role for such cells in the early phase of atherosclerosis.",

keywords = "Aorta, Arachidonic acid, Atherosclerosis, Lipoxygenase products, Smooth muscle cell migration",

author = "Junko Nakao and Toshiro Ooyama and Hideki Ito and Chang, {Wen Chang} and Murota, {Sei itsu}",

year = "1982",

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AU - Nakao, Junko

AU - Ooyama, Toshiro

AU - Ito, Hideki

AU - Chang, Wen Chang

AU - Murota, Sei itsu

PY - 1982/9

Y1 - 1982/9

N2 - We investigated the effects of mono-hydroxyeicosatetraenoic acids (HETEs) and N-formyl-methionyl-leucyl-phenylalanine (F-Met-Leu-Phe) on rat aortic smooth muscle cell migration in modified Boyden chambers. 12-HETE showed the most potent stimulatory effect on smooth muscle cell migration among the mono-HETEs tested. The optimal concentrations for cell migration were 3 × 10-15 and 3 × 10-13 g/ml for 12-HETE and 10-8 g/ml for 15-HETE. 5-HETE and F-Met-Leu-Phe were inactive with these cells. As 12-HETE is biosynthesized from arachidonic acid by the 12-lipoxygenase pathway in platelets and macrophages, and 15-HETE by the 15-lipoxygenase pathway in granulocytes, the present results indicate an important role for such cells in the early phase of atherosclerosis.

AB - We investigated the effects of mono-hydroxyeicosatetraenoic acids (HETEs) and N-formyl-methionyl-leucyl-phenylalanine (F-Met-Leu-Phe) on rat aortic smooth muscle cell migration in modified Boyden chambers. 12-HETE showed the most potent stimulatory effect on smooth muscle cell migration among the mono-HETEs tested. The optimal concentrations for cell migration were 3 × 10-15 and 3 × 10-13 g/ml for 12-HETE and 10-8 g/ml for 15-HETE. 5-HETE and F-Met-Leu-Phe were inactive with these cells. As 12-HETE is biosynthesized from arachidonic acid by the 12-lipoxygenase pathway in platelets and macrophages, and 15-HETE by the 15-lipoxygenase pathway in granulocytes, the present results indicate an important role for such cells in the early phase of atherosclerosis.

KW - Aorta

KW - Arachidonic acid

KW - Atherosclerosis

KW - Lipoxygenase products

KW - Smooth muscle cell migration

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JO - Atherosclerosis

JF - Atherosclerosis

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ER -

Comparative effect of lipoxygenase products of arachidonic acid on rat aortic smooth muscle cell migration

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ASJC Scopus subject areas

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