TY - JOUR
T1 - Color-indicating, label-free, dye-doped liquid crystal organic-polymer-based-bioinspired sensor for biomolecule immunodetection
AU - Huang, Haw Ming
AU - Chuang, Er Yuan
AU - Chen, Fu Lun
AU - Lin, Jia De
AU - Hsiao, Yu Cheng
N1 - Funding Information:
Funding: This work was financially supported by the Ministry of Science and Technology, Taiwan, under grant no. MOST109-2636-E-038-001, 108-2221-E-038-017-MY3 and 108-2320-B-038-061-MY3; by Taipei Medical University–Wan Fang Hospital, Taiwan under grant no. 109TMU-WFH-14, and by Taipei Medical University, Taiwan, under grant no. TMU106-AE1-B49.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/10
Y1 - 2020/10
N2 - The highly sensitive interfacial effects between liquid crystal (LC) and alignment layers make LC-bioinspired sensors an important technology. However, LC-bioinspired sensors are limited by quantification requiring a polarized microscope and expensive equipment, which makes it difficult to commercialize LC-bioinspired sensors. In this report, we first demonstrate that dye-doped LC (DDLC) chips coated with vertically aligned layers can be employed as a new LC-bioinspired sensing technology. The DDLC-bioinspired sensor was tested by detecting bovine serum albumin (BSA) and immunocomplexes of BSA pairs. The intensities of the dye color of the DDLC-bioinspired sensor can be changed with the concentrations of biomolecules and immunocomplexes. A detection limit of 0.5 µg/mL was shown for the color-indicating DDLC-bioinspired sensors. We also designed a new method to use the quantitative DDLC-bioinspired sensor with a smart-phone for potential of home test. The novel DDLC-bioinspired sensor is cheap, label-free, and easy to use, furthering the technology for home and field-based disease-related detection.
AB - The highly sensitive interfacial effects between liquid crystal (LC) and alignment layers make LC-bioinspired sensors an important technology. However, LC-bioinspired sensors are limited by quantification requiring a polarized microscope and expensive equipment, which makes it difficult to commercialize LC-bioinspired sensors. In this report, we first demonstrate that dye-doped LC (DDLC) chips coated with vertically aligned layers can be employed as a new LC-bioinspired sensing technology. The DDLC-bioinspired sensor was tested by detecting bovine serum albumin (BSA) and immunocomplexes of BSA pairs. The intensities of the dye color of the DDLC-bioinspired sensor can be changed with the concentrations of biomolecules and immunocomplexes. A detection limit of 0.5 µg/mL was shown for the color-indicating DDLC-bioinspired sensors. We also designed a new method to use the quantitative DDLC-bioinspired sensor with a smart-phone for potential of home test. The novel DDLC-bioinspired sensor is cheap, label-free, and easy to use, furthering the technology for home and field-based disease-related detection.
KW - Bovine serum albumin
KW - Dye-doped liquid crystal
KW - Label-free biosensor
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U2 - 10.3390/polym12102294
DO - 10.3390/polym12102294
M3 - Article
AN - SCOPUS:85092776487
SN - 2073-4360
VL - 12
SP - 1
EP - 10
JO - Polymers
JF - Polymers
IS - 10
M1 - 2294
ER -